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谢能中,石毓梅,李检秀,黄艳燕,杜奇石,黄日波.生物法合成乙偶姻手性异构体的研究进展[J].广西科学,2017,24(1):33-39. [点击复制]
- XIE Nengzhong,SHI Yumei,LI Jianxiu,HUANG Yanyan,DU Qishi,HUANG Ribo.Research Progress in Biological Production of Chiral Acetoin[J].Guangxi Sciences,2017,24(1):33-39. [点击复制]
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| 生物法合成乙偶姻手性异构体的研究进展 |
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谢能中1, 石毓梅2, 李检秀1, 黄艳燕1, 杜奇石1, 黄日波1,3
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| (1.广西科学院, 非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物质产业化工程院, 广西生物炼制重点实验室, 广西南宁 530007;2.钦州市产品质量监督检验所, 广西钦州 535008;3.广西大学生命科学与技术学院, 广西南宁 530004) |
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| 摘要: |
| 乙偶姻是一种重要的四碳平台化合物,在食品、医药、烟草、化妆品、化工等行业具有广泛的用途,其手性异构体(R)-乙偶姻和(S)-乙偶姻由于具有独特的立体结构,还可用于合成高附加值手性药物、化学中间体、液晶材料。本文综述了近年来利用酶法、全细胞催化法和基因工程菌发酵法制备手性乙偶姻的研究成果,介绍了利用代谢工程、合成生物学等技术在构建基因工程菌株,以及在提高乙偶姻产量、转化率和光学纯度方面所取得的研究进展。最后讨论了目前所存在的问题和难点,以及今后应该重点关注的研究方向。 |
| 关键词: (R)-乙偶姻 (S)-乙偶姻 手性合成 代谢工程 合成生物学 细胞工厂 |
| DOI：10.13656/j.cnki.gxkx.20170119.003 |
| 投稿时间：2016-10-12修订日期：2016-11-04 |
| 基金项目:广西科学研究与技术开发计划项目(14125008-2-22,14123001-19)和国家自然科学基金项目(21466007,31400079,31360207,31370716,31460296)资助 |
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| Research Progress in Biological Production of Chiral Acetoin |
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XIE Nengzhong1, SHI Yumei2, LI Jianxiu1, HUANG Yanyan1, DU Qishi1, HUANG Ribo1,3
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| (1.State Key Laboratory of Non-food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Qinzhou Products Quality Supervision and Inspection Institute, Qinzhou, Guangxi, 535008, China;3.College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| Acetoin is an important four carbon platform compound with a variety of applications in food, pharmacy, tobacco, cosmetics, and chemical synthesis.In addition, chiral acetoin including (R)-acetoin and (S)-acetoin has special applications in synthesis of optical pharmaceuticals, chemical intermediates, and liquid crystal composites.This article reviews recent advances in biological processes for chiral acetoin production, and highlights the metabolic engineering and synthetic biology approaches used to improve titers, yields, productivities, and optical purities.The challenges associated with current processes are discussed, and guidelines for future studies are proposed. |
| Key words: (R)-acetoin (S)-acetoin chiral synthesis metabolic engineering synthetic biology cell factory |
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