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杨立恩,韩晓磊,周伟,邓银银,许广平,胡传明,朱建一,陆勤勤,徐建国.条斑紫菜不同栽培品系的遗传多样性分析[J].广西科学,2016,23(3):241-247. [点击复制]
- YANG Li'en,HAN Xiaolei,ZHOU Wei,DENG Yinyin,XU Guangping,HU Chuanming,ZHU Jianyi,LU Qinqin,XU Jianguo.Genetic Diversity of Different Cultivars of Pyropia yezoensis[J].Guangxi Sciences,2016,23(3):241-247. [点击复制]
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| 条斑紫菜不同栽培品系的遗传多样性分析 |
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杨立恩1, 韩晓磊2, 周伟1, 邓银银1, 许广平1, 胡传明1, 朱建一2, 陆勤勤1, 徐建国3
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| (1.江苏省海洋水产研究所, 江苏南通 226007;2.常熟理工学院, 江苏常熟 215500;3.江苏省海洋渔业指挥部, 江苏南通 226000) |
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| 摘要: |
| [目的]确定通过杂交和突变选育出的条斑紫菜(Pyropia yezoensis)栽培品系的遗传多样性。[方法]采用简单重复序列区间(Inter simple sequence repeat,ISSR)、扩增片段长度多态性(Amplified fragment length polymorphism,AFLP)和随机扩增多态性DNA (Random amplified polymorphism DNA,RAPD)等3种分子标记对条斑紫菜不同栽培品系进行遗传多样性分析。[结果]3种分子标记的多态性比率分别为38.10%、38.95%和61.75%;所表现出来的不同栽培品系间遗传距离分别为0.1785,0.1029和0.2845;3种分子标记聚类结果并不完全一致,但可以看出Y-0602、Y-HA01和Y-H001总是聚类在一起,而Y-DL02倾向于单独聚为一支。[结论]通过诱变和杂交所选育的品系,在遗传角度上仍然属于条斑紫菜,但已形成不同栽培品系,具有一定的遗传多样性。 |
| 关键词: 条斑紫菜 遗传距离 分子标记 栽培品系 |
| DOI：10.13656/j.cnki.gxkx.20160713.012 |
| 投稿时间：2016-05-10修订日期：2016-06-15 |
| 基金项目:国家自然科学基金面上项目(31272664),国家海洋局海洋公益性行业科研专项项目(201105023),南通市科技计划项目(MS22015026)和江苏省水产三新工程重大项目(D2015-18)资助。 |
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| Genetic Diversity of Different Cultivars of Pyropia yezoensis |
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YANG Li'en1, HAN Xiaolei2, ZHOU Wei1, DENG Yinyin1, XU Guangping1, HU Chuanming1, ZHU Jianyi2, LU Qinqin1, XU Jianguo3
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| (1.Jiangsu Institute of Oceanology & Marine Fisheries, Nantong, Jiangsu, 226007, China;2.Changshu Institute of Technology, Changshu, Jiangsu, 215500, China;3.Marine Fisheries Dispatching Department of Jiangsu, Nantong, Jiangsu, 226000, China) |
| Abstract: |
| [Objective] Different cultivars were screened and bred by mutation or hybridization,and their genetic diversity was determined.[Methods] Molecular markers ISSR,AFLP and RAPD were employed to analyze the genetic diversity of screened cultivars.[Results] The polymorphism ratio shown by ISSR,AFLP and RAPD was 38.10%,38.95% and 61.75%,respectively.The genetic distance shown by ISSR,AFLP and RAPD between different cultivars of Pyropia yezoensis was 0.1785,0.1029 and 0.2845,respectively.Although the clustering results were not consistent,the cultivars Y-0602,Y-HA01 and Y-H001 always clustered together,whereas Y-DL02 tended to cluster as outgroup.[Conclusion] After being screened and bred by mutation or hybridization,the cultivars studied in the present paper are genetically the same species as P.yezoensis but essentially different cultivars with certain genetic diversity. |
| Key words: Pyropia yezoensis genetic distance molecular markers cultivars |
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