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王超,孙春晓,乔洪金,丛超,王际英,张利民.不同营养方式下小球藻生长与光合作用的变化[J].广西科学,2016,23(2):115-119. [点击复制]
- WANG Chao,SUN Chunxiao,QIAO Hongjin,CONG Chao,WANG Jiying,ZHANG Limin.Variation of Growth and Photosynthesis in Chlorella sorokiniana Under Different Trophic Modes[J].Guangxi Sciences,2016,23(2):115-119. [点击复制]
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| 不同营养方式下小球藻生长与光合作用的变化 |
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王超1, 孙春晓2, 乔洪金2, 丛超2,3, 王际英2, 张利民2
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| (1.青岛大学生命科学学院, 山东青岛 266071;2.山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东烟台 264006;3.上海海洋大学水产与生命学院, 上海 201306) |
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| 摘要: |
| [目的]研究小球藻在自养条件下和以乙酸为碳源的异养、混养条件下生长以及光合作用的变化。[方法]以小球藻Chlorella sorokiniana为研究对象,通过测定OD550和光系统Ⅱ(PSⅡ)叶绿素荧光研究其生长情况和光合作用的变化。[结果]小球藻在初始接种浓度为5×106个/mL的条件下,异养和混养的生长速度显著快于自养,到达稳定期仅需1.5d,而自养生长需要9d;叶绿素荧光参数自养大于混养,而混养又大于异养,有效光量子产量混养比自养降低21.5%,异养比自养降低98.1%;混养的Rubisco酶基因mRNA表达量最高,分别是异养和自养的3.2倍和1.8倍。[结论]小球藻在混养条件下光系统仅受到微弱抑制,生长速度和最高细胞密度均高于其它营养方式,适合规模化培养。 |
| 关键词: 小球藻 自养 异养 混养 光合作用 |
| DOI：10.13656/j.cnki.gxkx.20160512.001 |
| 投稿时间：2016-02-21修订日期：2016-04-05 |
| 基金项目:山东省优秀中青年科学家科研奖励基金项目(BS2013HZ018),国家自然科学基金青年基金项目(31201973),海洋生物产业——水生动物营养与饲料研发创新示范平台项目(201303003)和山东省科技发展计划项目(2014GHY115006)资助。 |
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| Variation of Growth and Photosynthesis in Chlorella sorokiniana Under Different Trophic Modes |
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WANG Chao1, SUN Chunxiao2, QIAO Hongjin2, CONG Chao2,3, WANG Jiying2, ZHANG Limin2
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| (1.College of Life Science, Qingdao University, Qingdao, Shandong, 266071, China;2.Key Laboratory of Marine Ecological Restoration, Shandong Marine Resource and Environment Research Institute, Yantai, Shandong, 264006, China;3.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China) |
| Abstract: |
| [Objective] The variation of growth and photosynthesis of microalgae was studied and compared under autotrophic condition and hetero- and mixotrophic conditions with acetate as carbon source.[Methods] With Chlorella sorokiniana as material, the changes of growth and photosynthesis were reflected by determining the OD550 and the chlorophyll fluorescence from photosystem Ⅱ (PS Ⅱ).[Results] The growth rates of hetero- and mixotrophic conditions were significantly higher than that of autotrophic condition.It was only 1.5d for cells reaching stationary phase under hetero- and mixotrophic conditions, but 9d for cells under autotrophic condition.The parameters of chlorophyll fluorescence under autotrophic condition were significantly higher than that under hetero- and mixotrophic conditions, and that under mixotrophic condition were significantly higher than that under heterotrophic condition.The effective PS Ⅱ quantum yield under mixo- and heterotrophic condition was reduced 21.5% and 98.1%, respectively, compared with that of autotrophic condition.The mRNA expression of Rubisco gene under mixotrophic condition were highest, and 3.2-fold and 1.8-fold of that under hetero- and autotrophic conditions, respectively.[Conclusion] The photosystems of Chlorella under mixotro-phic condition only slightly inhibited.In addition, the growth rate and maximal cell density under mixotrophic condition were higher than other conditions.Therefore, mixotrophic growth mode is suitable for large-scale cultivation. |
| Key words: Chlorella sorokiniana autotrophic heterotrophic mixotrophic photosynthesis |
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