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李亿,李检秀,刘海余,黄艳燕,陆琦,谢能中,黄日波.多粘类芽孢杆菌同步糖化发酵玉米粉生产(R,R)-2,3-丁二醇[J].广西科学,2016,23(1):41-46. [点击复制]
- LI Yi,LI Jianxiu,LIU Haiyu,HUANG Yanyan,LU Qi,XIE Nengzhong,HUANG Ribo.Simultaneous Sccharification and(R,R)-2,3-butanediol Fermentation from Corn Flour by Paenibacillus polymyxa[J].Guangxi Sciences,2016,23(1):41-46. [点击复制]
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| 多粘类芽孢杆菌同步糖化发酵玉米粉生产(R,R)-2,3-丁二醇 |
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李亿1,2, 李检秀1,2, 刘海余1, 黄艳燕2, 陆琦2, 谢能中2, 黄日波1,2
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| (1.广西大学生命科学与技术学院, 广西南宁 530005;2.广西科学院, 非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物炼制重点实验室, 广西生物质产业化工程院, 广西南宁 530007) |
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| 摘要: |
| [目的]对多粘类芽孢杆菌Paenibacillus polymyxa的玉米粉同步糖化发酵工艺进行优化,以获得低成本、高效的(R,R)-2,3-丁二醇生产技术。[方法]研究玉米粉浓度、氮源种类和氮源浓度对菌体生长、耗糖能力以及(R,R)-2,3-丁二醇产量、产率、得率和转化率的影响,并在此基础上进一步考察培养基中其它成分对(R,R)-2,3-丁二醇发酵的影响。[结果]优化后的培养基组分为玉米干粉140g/L,酵母粉30g/L,Na2HPO4 3g/L,KH2PO4 3g/L,(NH4)2SO4 2g/L,MgSO4 0.8g/L,微量元素溶液2mL/L。使用优化后的培养基进行同步糖化发酵,发酵50h后(R,R)-2,3-丁二醇产量达到56.28g/L (光学纯度为98.3%),对葡萄糖的得率为0.44g/g,产率为1.13g/(L·h)。[结论](R,R)-2,3-丁二醇生产技术低价高效,可为其工业化生产提供参考。 |
| 关键词: (R,R)-2,3-丁二醇 多粘类芽孢杆菌 同步糖化 发酵工艺 低价碳源 |
| DOI：10.13656/j.cnki.gxkx.20160315.006 |
| 投稿时间：2015-11-13修订日期：2016-01-26 |
| 基金项目:国家自然科学基金项目(31400079,21466007,31160023),中科院微生物生理与代谢工程重点实验室开放课题(KLIM-201304)和广西科学研究与技术开发计划项目(桂科合14125008-2-22)资助。 |
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| Simultaneous Sccharification and(R,R)-2,3-butanediol Fermentation from Corn Flour by Paenibacillus polymyxa |
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LI Yi1,2, LI Jianxiu1,2, LIU Haiyu1, HUANG Yanyan2, LU Qi2, XIE Nengzhong2, HUANG Ribo1,2
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| (1.College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China;2.State Key Laboratory of Non-food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objectives] In this study, corn flour simultaneous saccharification and fermentationprocess was optimized to achieve a low-cost and efficient (R, R)-2, 3-butanediol production technology.[Methods] The effects of different types and concentrations of nitrogen, and different concentrations of corn flour on cell growth, consumption of sugar, (R, R)-2, 3-butanediol production, productivity, yield and conversion rate were investigated based on corn flour simultaneous saccharification and fermentation.Furthermore, the effects of other components in the medium on (R, R)-2, 3-butanediol fermentation were observed.[Results] The optimized composi-tion of culture medium was as follows:140g/L dried corn flour, 30g/L yeast extract, 3g/L Na2HPO4, 3g/L KH2PO4, 2g/L (NH4)2SO4, 0.8g/L MgSO4 and 2mL/L trace element solution.Under abovementioned conditions, up to 56.28g/L (R, R)-2, 3-butanediol (optical purity >98.3%)could be achieved within 50 hwith the yield on glucose of 0.44g/g and the conversion rate of 1.13g/(L·h).This is the highest production of (R, R)-2, 3-butanediol titer so far reported from low-cost carbon source.[Conclusion] This study develops a technology utilizing low-cost carbon to produce (R, R)-2, 3-butanediol efficiently, which provides meaningful guidance for its future industrial production. More |
| Key words: (R,R)-2,3-butanediol Paenibacillus polymyxa simultaneous saccharification fermentation process low-cost carbon source |
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