| 摘要: |
| [目的]利用光镊拉曼光谱技术(Laser Tweezers Raman Spectroscopy,LTRS)研究金黄色葡萄球菌(Staphylococcus aureus)细胞内主要生物大分子含量的动态变化,探寻监测微生物代谢状态变化的有效工具。[方法]收集分批培养过程不同时间点金黄色葡萄球菌细胞的拉曼光谱,观察其特征峰依赖时间的变化特点。[结果]归属于核酸的特征峰,如667cm-1峰,724cm-1峰,781cm-1峰,809cm-1峰和1 574cm-1峰在整个分批生长阶段持续降低,在对数生长期下降幅度尤其剧烈,反映了对数生长期细胞分裂快速,DNA复制活跃;与蛋白质相关的特征峰,如850cm-1峰,1 001cm-1峰和1 662cm-1峰,在对数生长期显著增强,而到稳定期峰强度基本维持恒定。[结论]光镊拉曼光谱技术能实时监测生物样品内生物大分子的含量,是单细胞水平上研究微生物代谢状态变化的有效工具。 |
| 关键词: 金黄色葡萄球菌 拉曼光谱 激光镊子 单细胞分析 |
| DOI：10.13656/j.cnki.gxkx.20160315.009 |
| 投稿时间：2015-09-25 |
| 基金项目:广西自然科学基金项目(2014GXNSFAA118193)和广西科学院基本科研业务费项目(15YJ22SL08)资助。 |
|
| Study on Bio-Macromolecular Level Changes in Staphylococcus aureus Cells during Batch Cultivation Using Laser Tweezers Raman Spectroscopy |
|
TAO Zhanhua1, SUN Meijuan2
|
| (1.Laboratory of Biophysics of Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Department of Physics and Telecommunication Engineering of Baise University, Baise, Guangxi, 533000, China) |
| Abstract: |
| [Objective] The dynamic change in content of major biological molecules inside Staphylococcus aureus cells in batch cultivation was investigated using laser tweezers Raman spectroscopy.[Methods] Raman spectra of Staphylococcus aureus cells sampled at various time points of batch cultivation were recorded to observe time-dependent changes of the characteristic peaks.[Results] The intensities of characteristic bands at 667cm-1, 724cm-1, 781cm-1, 809cm-1 and 1 574cm-1, assigned to nucleic acids, decreased continuously throughout the batch growth, and the intensities of these bands dropped even more drastically, especially during the exponential growth phase, which could be attributed to the rapid cell division and active DNA replication in the exponential growth stage.In the meantime, the intensities of Raman peaks around 850cm-1, 1 001cm-1 and 1 662cm-1, associated with proteins, increased significantly during the exponential growth phase, and then remained relatively constant during the stationary phase.[Conclusion] The experimental results show that laser tweezers Raman spectroscopy can monitor the content of bio-macromolecules in real time and serve as an efficient tool for analyzing the changes in metabolic states of microorganisms at single cell level. |
| Key words: Staphylococcus aureus Raman spectroscopy laser tweezers single cell analysis |
