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梁乔芳,刘华钢,谭强,苏桂玉,彭晓丽,徐红娟.二去水卫矛醇对人脑肿瘤细胞体外抑制作用[J].广西科学,2015,22(4):454-456. [点击复制]
- LIANG Qiao-fang,LIU Hua-gang,TAN Qiang,SU Gui-yu,PENG Xiao-li,XU Hong-juan.Inhibitory Effect of 1,2: 5,6-Dianhydrogalctitol on Human Brain Tumor Cells in Vitro[J].Guangxi Sciences,2015,22(4):454-456. [点击复制]
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| 二去水卫矛醇对人脑肿瘤细胞体外抑制作用 |
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梁乔芳1, 刘华钢2, 谭强1, 苏桂玉1, 彭晓丽1, 徐红娟1
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| (1.广西中医药大学药学院, 广西南宁 530001;2.广西医科大学药学院, 广西南宁 530021) |
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| 摘要: |
| [目的]探寻二去水卫矛醇(1,2:5,6-Dianhydrogalctitol,DAG)对人脑肿瘤细胞的抑制作用,为DAG抗肿瘤作用提供理论基础。[方法]通过体外实验,并采用噻唑蓝(MTT)比色法检测不同浓度DAG对11株人脑肿瘤细胞的体外抑制作用,观察DAG的半数抑制浓度。[结果] DAG对IMR-32、SH-SY5Y、U87、A172、BT325、T98G、SHG-44、U373、C6、SK-N-BE、U251等11株人脑肿瘤细胞作用72 h后半数抑制浓度(72h-IC50)分别为24.28 μg/mL、19.24 μg/mL、21.55 μg/mL、25.13 μg/mL、20.63 μg/mL、21.03 μg/mL、24.91 μg/mL、23.52 μg/mL、22.06 μg/mL、22.92 μg/mL、23.44 μg/mL。[结论] DAG在体外对上述11株人脑细胞均有抑制生长作用。 |
| 关键词: 二去水卫矛醇 人脑肿瘤细胞 噻唑蓝试验 半数抑制浓度 |
| DOI： |
| 投稿时间：2015-03-24修订日期：2015-04-25 |
| 基金项目:广西科学研究与技术开发计划项目(桂科能12237007)资助。 |
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| Inhibitory Effect of 1,2: 5,6-Dianhydrogalctitol on Human Brain Tumor Cells in Vitro |
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LIANG Qiao-fang1, LIU Hua-gang2, TAN Qiang1, SU Gui-yu1, PENG Xiao-li1, XU Hong-juan1
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| (1.Pharmacy College, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530001, China;2.Pharmacy College, Guangxi Medical University, Nanning, Guangxi, 530021, China) |
| Abstract: |
| [Objective] The inhibitory effect of 1, 2:5, 6-Dianhydrogalctitol(DAG) on 11 strains of human brain tumor cells was investigated in vitro.[Methods] MTT assay was performed to determine the half inhibitory concentration of DAG for the 11 strains of human brain tumor cells.[Results] The half inhibitory concentration of DAG for IMR-32、SH-SY5Y, U87, A172, BT325, T98G, SHG-44, U373, C6, SK-N-BE and U251 within 72 h was 24.28 μg/mL, 19.24 μg/mL, 21.55 μg/mL, 25.13 μg/mL, 20.63 μg/mL, 21.03 μg/mL, 24.91 μg/mL, 23.52 μg/mL, 22.06 μg/mL, 22.92 μg/mL and 23.44 μg/mL, respectively.[Conclusion] DAG exhibits the inhibitory activity on the above mentioned 11 strains of human brain tumor cells in vitro. |
| Key words: 1,2:5,6-Dianhydrogalctitol human brain tumor cells MTT assay the half inhibitory concentration |
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