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苏志维,陈波,何碧娟,高程海,王一兵,马仲辉.双肾藤茎枝甾体类化学成分的分离鉴定[J].广西科学,2015,22(4):445-448,453. [点击复制]
- SU Zhi-wei,CHEN Bo,HE Bi-juan,GAO Cheng-hai,WANG Yi-bing,MA Zhong-hui.Separation and Identification of Steroids from the Stem of Bauhinia glauca subsp.hupehana[J].Guangxi Sciences,2015,22(4):445-448,453. [点击复制]
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| 双肾藤茎枝甾体类化学成分的分离鉴定 |
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苏志维1, 陈波1, 何碧娟1, 高程海1, 王一兵1, 马仲辉2
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| (1.广西科学院广西北部湾海洋研究中心, 广西南宁 530007;2.广西大学农学院, 广西南宁 530004) |
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| 摘要: |
| [目的]系统研究药用植物双肾藤(Bauhinia glauca subsp.hupehana)茎枝的化学成分,明确其药用物质基础。[方法]采用硅胶柱层析、聚酰胺柱层析、Sephadex LH-20凝胶柱层析等分离技术,从双肾藤茎枝中分离甾体类单体化合物,运用理化和波谱分析方法鉴定其化学结构。[结果]从双肾藤茎枝分离获得6个甾体类化合物,分别鉴定为(24R)-5α-豆甾烷-3,6-二酮(1)、(22E,24R)-麦角甾-4,6,8(14),22-四烯-3-酮(2)、(24R)-5α-豆甾-4-en-3,6-二酮(3)、豆甾烷-4-烯-3-酮(4)、β-谷甾醇(5)、胡萝卜苷(6)。[结论]从双肾藤中分离获得的甾体类化合物包括甾醇、甾酮和甾体苷,其中化合物1和2均为首次从该属植物中分离得到。 |
| 关键词: 双肾藤 甾体类化合物 分离纯化 结构鉴定 |
| DOI： |
| 投稿时间：2015-03-26修订日期：2015-05-15 |
| 基金项目:广西科学院基本科研业务费项目(15YJ22HY06)和广西自然科学基金项目(2012GXNSFEA053001)资助。 |
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| Separation and Identification of Steroids from the Stem of Bauhinia glauca subsp.hupehana |
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SU Zhi-wei1, CHEN Bo1, HE Bi-juan1, GAO Cheng-hai1, WANG Yi-bing1, MA Zhong-hui2
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| (1.Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| [Objective] The chemical constituents from the stem of medicinal plant Bauhinia glauca subsp.hupehana were systematically investigated in order to determine its medicinal materials.[Methods] The compounds were isolated and purified by using a combination of various chromatographic techniques including column chromatography over silica gel, polyamide and Sephadex LH-20 gel, and their structures were identified by physicochemical and spectral analyses.[Results] Detailed chemical constituents investigation of the stem of B.glauca subsp.hupehana led to the isolation of six steroids compounds by various chromatographic methods, whose structures were fully determined as(24R)-5α-stigmastane-3, 6-dione (1), (22E, 24R)-ergosta-4, 6, 8(14), 22-tetraen-3-one (2), (24R)-5α-stigmast-4-en-3, 6-dione (3), stigmast-4-en-3-one (4) and β-sitosterol (5), β-daucosterol (6).[Conclusion] The steroids isolated from the stem of B.glauca subsp.hupehana comprise sterols, sterones and steroid glycosides, of which the first two were obtained from genus Bauhinia for the first time. |
| Key words: Bauhinia glauca subsp.hupehana steroids isolation and purification structural |
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