| 引用本文： |
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陆登俊,何亮亮,刘桂云,黄琳娟,李坚斌,梁欣泉.蔗渣木聚糖制备低聚木糖的最优复合酶降解工艺研究[J].广西科学,2014,21(6):610-613. [点击复制]
- LU Deng-jun,HE Liang-liang,LIU Gui-yun,HUANG Lin-juan,LI Jian-bin,LIANG Xin-quan.The Research on Optimal Production Process of Xylooligosaccharides by Composite Enzymatic Hydrolysis of Bagasse Xylan[J].Guangxi Sciences,2014,21(6):610-613. [点击复制]
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| 摘要: |
| [目的]研究复合酶酶解蔗渣木聚糖制备低聚木糖的方法。[方法]采用混料试验设计中的单纯形质心方法对木聚糖酶A,木聚糖酶B和α-L-阿拉伯糖苷酶3种酶进行配方设计试验,以低聚木糖得率为评价指标。[结果]低聚木糖得率的最优酶复合配比为木聚糖酶A 39.5%,木聚糖酶B 25%,α-L-阿拉伯糖苷酶35.5%,在此条件下预测低聚木糖得率为85.20%。[结论]该配比能显著提高蔗渣木聚糖酶解效率和低聚木糖的产率。 |
| 关键词: 复合酶 蔗渣木聚糖 低聚木糖 蔗渣 |
| DOI： |
| 投稿时间：2014-09-30修订日期：2014-11-01 |
| 基金项目:广西科学基金项目(桂科青0832009)和2011广西大学实验教学示范中心精品、特色项目建设项目资助。 |
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| The Research on Optimal Production Process of Xylooligosaccharides by Composite Enzymatic Hydrolysis of Bagasse Xylan |
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LU Deng-jun1, HE Liang-liang2, LIU Gui-yun1, HUANG Lin-juan1, LI Jian-bin1, LIANG Xin-quan1
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| (1.Guangxi University College of Light Industry and Food Engineering, Nanning, Guangxi, 530004, China;2.Science Research Institute of Light Industry, Kunming, Yunnan, 650034, China) |
| Abstract: |
| [Objective] The production of xylooligosaccharides was studied by composite enzymatic hydrolysis of bagasse xylan.[Methods] The formulation of xylanase A, xylanase B and alpha-L-arabinofuranosidase was opitimized through simplex-centroid method for mixture design experiments, with the XOS extraction rate as evaluation index.[Results] The best formula was determined as:Xylanase A 39.5%, xylanase B 25% and alpha-L-arabinofuranosidase 35.5%.Under these conditions, the extraction rate of XOS was 85.20%.[Conclusion] The optimization rate of compound enzyme has the characteristic of increasing yield and efficiency. |
| Key words: complex xylanase bagasse xylan xylooligosaccharide bagasse |
