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  • 龙彬,高程海,胡丽琴,潘丽霞,林琳,余炼,文良娟.柳珊瑚共生菌Bucillus methylotrophicus发酵液化学成分研究[J].广西科学,2014,21(1):89-92.    [点击复制]
  • LONG Bin,GAO Cheng-hai,HU Li-qin,PAN Li-xia,LIN Lin,YU Lian,WEN Liang-juan.Study on Chemical Constituents from Marine Gorgonian-associated Bacterium Bucillus methylotrophicus[J].Guangxi Sciences,2014,21(1):89-92.   [点击复制]
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柳珊瑚共生菌Bucillus methylotrophicus发酵液化学成分研究
龙彬1,2, 高程海1, 胡丽琴1,2, 潘丽霞1, 林琳1,2, 余炼1,2, 文良娟2
0
(1.广西科学院广西近海海洋环境科学重点实验室, 广西南宁 530007;2.广西大学轻工与食品工程学院, 广西南宁 530004)
摘要:
[目的]研究柳珊瑚Anthogorgia caerulea共生细菌Bacillus methylotrophicus发酵液中的化学成分。[方法]采用多种柱色谱和高效液相色谱分离技术,对柳珊瑚A.caerulea共生菌B.methylotrophicus发酵液中化学成分进行研究,运用理化和波谱分析方法鉴定其化学结构。[结果]从共生细菌B.Methylotrophicus发酵液中分离了6个化合物,分别鉴定为环(脯-甘)二肽(1)、环(S-脯-R-亮)二肽(2)、胸苷(3)、尿苷(4)、原儿茶酸(5)、3-Furancarboxylic acid-5-(hydroxmenthy)-Furan(6)。[结论]化合物1~6均是首次从该种细菌中分离得到,丰富了柳珊瑚共生菌B.methylotrophicus的化学多样性。
关键词:  柳珊瑚  海洋细菌  化合物  结构鉴定
DOI:
投稿时间:2013-11-01修订日期:2013-12-09
基金项目:国家自然科学基金项目(81260480),广西自然科学基金项目(2011GXNSFB018035,2011GXNSFE018002,2012GXNSFAA-053160,2012GXNSFEA053001)资助。
Study on Chemical Constituents from Marine Gorgonian-associated Bacterium Bucillus methylotrophicus
LONG Bin1,2, GAO Cheng-hai1, HU Li-qin1,2, PAN Li-xia1, LIN Lin1,2, YU Lian1,2, WEN Liang-juan2
(1.Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China)
Abstract:
[Objective] To investigate the chemical constituents of the fermentation liquid from marine gorgonian-associated bacterium Bucillus methylotrophicus.[Method] The compounds were isolated and purified by column chromatography on silica gel and their structures were identified by spectral analyses and comparison with literatures.[Result] Six compounds were obtained from the fermentation liquid of B.methylotrophicus and characterized as Cyolo-(Pro-Gly) (1), Cyolo-(S-pro-R-leu) (2), Thymidine (3), Uridine (4), Protocatechuic acid (5), 3-Furancarboxylic acid-5-(hydroxmenthy)-Furan (6).[Conclusion] Compounds 1~6 were isolated from B.methylotrophicus for the first time, which enriches the chemical diversity of marine gorgonian-associated bacterium Bucillus methylotrophicus.
Key words:  gorgonian  marine bacteria  compouds  chemical identification

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