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吴仁智,陈东,芦志龙,陆琦,张穗生,黄日波.发酵木糖高产乙醇树干毕赤酵母菌株的Co60诱变选育[J].广西科学,2014,21(1):47-53. [点击复制]
- WU Ren-zhi,CHEN Dong,LU Zhi-long,LU Qi,ZHANG Sui-sheng,HUANG Ri-bo.Breeding of High-yield Pichia stipitis Strains by Co60 Mutagenesis for Ethanol Fermentation from Xylose[J].Guangxi Sciences,2014,21(1):47-53. [点击复制]
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| 发酵木糖高产乙醇树干毕赤酵母菌株的Co60诱变选育 |
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吴仁智1,2, 陈东1,2, 芦志龙1, 陆琦1, 张穗生1, 黄日波1,2
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| (1.广西科学院, 非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物质产业化工程院, 广西生物炼制重点实验室, 广西南宁 530007;2.广西大学生命科学与技术学院, 广西南宁 530004) |
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| 摘要: |
| [目的]选育能够利用木糖高产乙醇的酵母菌株。[方法]采用Co60诱变树干毕赤酵母(Pichia stipitis),筛选乙醇产量高的突变菌株,并对原始菌株、突变菌株的生长发酵特性和两个菌株对高浓度木糖、乙醇的耐受性进行比较。[结果]在YPX培养基上筛选获得1株能够高效发酵木糖的突变菌株1K-9。该菌株在50mL 15% FM发酵84h,发酵液乙醇含量最高达(51.034±0.112)g/L,比原始菌株提高10.05%;在500mL 15% FM发酵96h,乙醇含量最高达(51.390±0.119)g/L;在500mL 20% FM发酵156h,乙醇含量最高达(52.496±0.513)g/L。菌株1K-9在HSM培养基或含4%~5%乙醇的YPX培养基中生长良好,在含6%~7%乙醇的YPX培养基中生长缓慢。[结论]Co60诱变对于树干毕赤酵母(Pichia stipitis)菌株是有效的,能选育出木糖高产乙醇酵母菌株1K-9。 |
| 关键词: 诱变 树干毕赤酵母 乙醇 木糖 选育 |
| DOI： |
| 投稿时间：2013-07-29修订日期：2013-09-30 |
| 基金项目:国家973项目(2010CB736209,2012CB723605),国家863项目(2012AA022106,2012AA023406,2012AA022302,2013AA050701),国家国际合作项目(2010DFB63590,2011DFA61910),广西科学研究与技术开发计划项目(桂科合10100019-21,桂科攻1099071,桂科合1140010-15),广西科技创新能力与条件建设计划项目(桂科能12237022),广西自然科学基金项目(2012GXNSFAA053062),广西科学院基本科研业务费项目(10YJ25SW15,12YJ25SW04),八桂学者建设工程专项经费项目,广西生物质产业化工程院建设(桂科能12237022),广西生物炼制重点实验室项目(13-051-08)资助。 |
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| Breeding of High-yield Pichia stipitis Strains by Co60 Mutagenesis for Ethanol Fermentation from Xylose |
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WU Ren-zhi1,2, CHEN Dong1,2, LU Zhi-long1, LU Qi1, ZHANG Sui-sheng1, HUANG Ri-bo1,2
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| (1.Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;2.College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China) |
| Abstract: |
| [Objective] To breed high-yield ethanol strains, which can use xylose in fermentation, [Method] xylose-fermenting yeast Pichia stipitis was mutated by Co60 rays. The growth and fermentation characteristics of original and mutant strains were analyzed, and their tolerances against relatively high concentrations of xylose and ethanol were compared.[Result] The mutant 1K-9, which shows superior xylose utilizing, growing and ethanol producing efficiency was screened on YPX.After fermenting in 15%FM for 84h, the concentration of ethanol reached(51.034±0.112)g/L, increased by 10.05% comparing to the original strain.When amplifying fermentation with strain 1K-9 was conducted in 500mL medium, 15%FM and 20%FM could produce the concentration of ethanol reaching (51.390±0.119)g/L and (52.496±0.513)g/L after fermenting for 96h and 156h, respectively.The mutant strain 1K-9 could grow easily and quickly in HSM or in YPX containing 4%~5% ethanol, but hard in YPX containing 6%~7% ethanol. [Conclusion] Mutagenesis of Pichia stipitis can be effectively conducted by Co60 rays, through which the mutant strain 1K-9 was breed out and it can yield high production of ethanol from fermenting xylose. |
| Key words: mutagenesis Pichia stipitis ethanol xylose breeding |
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