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熊雅兰,晏伟,张穗生,陈东,陆琦,黄日波.酿酒酵母呼吸突变株的高糖胁迫反应研究[J].广西科学,2014,21(1):34-41. [点击复制]
- XIONG Ya-lan,YAN Wei,ZHANG Sui-sheng,CHEN Dong,LU Qi,HUANG Ri-bo.The Response of a Saccharomyces cerevisiae Respiratory Mutant to High Sugar Stress[J].Guangxi Sciences,2014,21(1):34-41. [点击复制]
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| 酿酒酵母呼吸突变株的高糖胁迫反应研究 |
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熊雅兰1, 晏伟1, 张穗生2, 陈东1,2, 陆琦2, 黄日波1,2
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| (1.广西大学生命科学与技术学院, 广西南宁 530004;2.广西科学院, 非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物质产业化工程院, 广西生物炼制重点实验室, 广西南宁 530007) |
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| 摘要: |
| [目的]研究野生型甘蔗糖蜜发酵高产乙醇菌株MF1002及其糖分利用能力显著提高的呼吸突变菌株MF15c在高糖胁迫下的生理特性变化。[方法]测定在高糖胁迫下菌株的生长速率、出芽率、乙醇产量和超氧化物歧化酶(SOD)活力、过氧化氢酶活力、过氧化物酶活力以及细胞质和线粒体的ATP酶活力。[结果]在葡萄糖浓度分别为25%、30%和40%的高糖培养基中,MF15c菌株生长和乙醇发酵受抑制的程度均明显低于MF1002。当葡萄糖浓度为30%和40%时,MF15c的最大菌体数目、最高出芽率和乙醇发酵浓度等均显著高于MF1002。当葡萄糖浓度为30%时,两菌株胞内的SOD活力、过氧化氢酶活力、过氧化物酶活力以及细胞质和线粒体的ATP酶活力均显著上升。其中,MF15c的胞内SOD活力、胞内过氧化物酶活力、细胞质ATP酶活力和线粒体ATP酶活力在高糖胁迫下的上升幅度显著高于MF1002。[结论]MF15c较MF1002具有更强的高糖耐受能力。SOD活力、过氧化氢酶活力、过氧化物酶活力以及细胞质和线粒体的ATP酶均参与了两菌株的高糖胁迫反应,胞内SOD活力、胞内过氧化物酶活力、细胞质ATP酶活力和线粒体ATP酶活力可能与MF15c菌株的高糖耐受能力有关,可作为进一步改造该菌株的指导指标。 |
| 关键词: 酿酒酵母 呼吸突变体 高糖胁迫 生理特性 |
| DOI： |
| 投稿时间：2013-05-21修订日期：2013-06-04 |
| 基金项目:国家973项目(2010CB736209),国家863项目(2012AA022106,2013AA050701),国家国际合作项目(2010DFB63590,2011DFA61910),广西科学研究与技术开发计划项目(桂科合10100019-21,桂科攻1099071,桂科合1140010-15),广西自然科学基金项目(2012GXNSFAA053062,2011GXNSFA018113),广西科学院基本科研业务费项目(12YJ25SW03),广西八桂学者建设工程专项经费等资助。 |
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| The Response of a Saccharomyces cerevisiae Respiratory Mutant to High Sugar Stress |
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XIONG Ya-lan1, YAN Wei1, ZHANG Sui-sheng2, CHEN Dong1,2, LU Qi2, HUANG Ri-bo1,2
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| (1.College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China;2.Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] The wild type Saccharomyces cerevisiae strain MF1002 that was a high yield strain of molasses ethanol fermentation, and its respiratory mutant MF15c of which the sugar utilizing capacity was significantly enhanced, were employed to investigate the physiologic response to the high sugar stress.[Method] The strains' growth rate, budding ratio, ethanol yield and enzyme activity of superoxide dismutase (SOD), catalase, peroxidase, ATPase activity in cytoplasm and the ATPase activity in mitochondria were conducted under high sugar stress.[Result] When the strains were cultivated in the medium containing 25%, 30% and 40% glucose, the growth and ethanol fermentation of MF1002 were repressed significantly stronger than MF15c by high sugar stress.When the glucose concentration in medium was increased to 30% and 40%, the maximum cell number, the maximum cell budding ratio and the ethanol fermentation concentration of MF15c were all significantly higher than that of MF1002.The enzymes activity assay exhibited that the activities of five enzyme afore mentioned were all significantly increased for both strains after they were cultivated in the medium containing 30% glucose for 24h under high sugar stress, of which the activity of 4 enzymes increased significant higher in MF15c than in MF1002, except for the catalase activity in cell extract.[Conclusion] MF15c has stronger tolerance than MF1002 to high sugar stress.Thus the activity of these 5 enzymes was involved in the response of both strains to high sugar stress, among which 4 enzyme activities may be correlated with the high sugar tolerance of MF15c and could be used as the indicator for further improvement of the strain. |
| Key words: Saccharomyces cerevisiae respiratory mutant sugar stress physiological characters |
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