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黄园,陈晓军,梁霞,廖厚知,庞继梅,廖九明.HPLC法测定参芪首乌补汁中2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量[J].广西科学,2013,20(4):282-284. [点击复制]
- HUANG Yuan,CHEN Xiao-jun,LIANG Xia,LIAO Hou-zhi,PANG Ji-mei,LIAO Jiu-ming.HPLC Determination of 2,3,5,4'-tetrahydroxystibene-2-O-β-D-glucoside in Shenqi Shouwu Buzhi[J].Guangxi Sciences,2013,20(4):282-284. [点击复制]
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| HPLC法测定参芪首乌补汁中2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量 |
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黄园, 陈晓军, 梁霞, 廖厚知, 庞继梅, 廖九明
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| (广西博科药业有限公司, 广西南宁 530007) |
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| 摘要: |
| 建立一种测定参芪首乌补汁中2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷(简称二苯乙烯苷)含量的方法。采用岛津VP-ODS色谱柱(150 mm×4.6mm,5m);乙腈-水(25:75)为流动相;检测波长为320nm,流速1.0mL·min-1。结果表明:二苯乙烯苷在0.1013~1.013μg范围内与峰面积呈良好线性关系,r=0.9999,平均加样回收率为98.84%,RSD为0.87%(n=6),精密度、稳定性良好。该方法可靠性高,分离度、准确性、重复性好,适用于参芪首乌补汁中二苯乙烯苷含量的测定。 |
| 关键词: 高效液相色谱法 参芪首乌补汁 2,3,5,4'-四羟基二苯乙烯-2-O-β-D葡萄糖苷 |
| DOI： |
| 投稿时间：2013-05-12修订日期：2013-09-10 |
| 基金项目: |
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| HPLC Determination of 2,3,5,4'-tetrahydroxystibene-2-O-β-D-glucoside in Shenqi Shouwu Buzhi |
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HUANG Yuan, CHEN Xiao-jun, LIANG Xia, LIAO Hou-zhi, PANG Ji-mei, LIAO Jiu-ming
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| (Guangxi Boke Pharmaceutical CO., LTD., Nanning, Guangxi, 530007, China) |
| Abstract: |
| The HPLC determination method was used to detect the 2, 3, 5, 4'-tetrahydroxystibene-2-O-β-D-glucoside in Shenqi Shouwu Buzhi by Shimadzu VP-ODS (4.6 mm×150mm, 5μm) chromatographic column.The mobile phase was a mixture of acetonitrile-water (25:75), the flow rate was 1mL·min-1.The detector wavelength was 320 nm.The precision and stability were fine in those conditions.The calibration curve was linear over the range of 0.1013~1.013μg for 2, 3, 5, 4'-tetrahydroxystibene-2-O-β-D-glucoside (r=0.9999), The average recovery rate (n=6) was 98.84%.The proposed method may be used in the quantitative analysis of 2, 3, 5, 4'-tetrahydroxystibene-2-O-β-D-glucoside in Shenqi Shouwu Buzhi with high reliability, separation, accuracy and repeatability. |
| Key words: HPLC Shenqi Shouwu Buzhi 2,3,5,4'-tetrahydroxystibene-2-O-β-D-glucoside |
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