| 引用本文： |
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方燕,潘丽霞,易湘茜,林琳,王一兵,高程海.柳珊瑚Anthogorgia caerulea相关可培养细菌抗污活性筛选[J].广西科学,2012,19(3):253-256. [点击复制]
- FANG Yan,PAN Li-xia,YI Xiang-xi,LIN Lin,WANG Yi-bing,GAO Cheng-hai.Antifouling Activity of Culturable Bacteria Associated with the Gorgonian Anthogorgia caerulea[J].Guangxi Sciences,2012,19(3):253-256. [点击复制]
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| 摘要: |
| 用4种不同培养基分离纯化柳珊瑚Anthogorgia caerulea中的相关可培养细菌。对获得的90株细菌,先用4株海洋污损指示菌进行活性初筛,获得11株具有抑制海洋污损指示菌生长的细菌后,再用2种抗海洋污损生物幼虫进行活性复筛,复筛得到5株对海洋污损生物幼虫附着有抑制活性的细菌。这5株细菌经16S rRNA基因序列分析,确定其系统发育地位为芽胞杆菌属(Bacillus sp.)。 |
| 关键词: 柳珊瑚 Anthogorgia caerulea 海洋细菌 抗污损生物活性 |
| DOI： |
| 投稿时间：2011-12-13修订日期：2012-03-16 |
| 基金项目:广西科学院基本业务经费(10YJ25HY02),广西青年基金项目(2011GXNSFB018035),广西科技攻关项目(桂科攻1114011-7),广西自然基金重大专项(2011GXNSFE018002)资助。 |
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| Antifouling Activity of Culturable Bacteria Associated with the Gorgonian Anthogorgia caerulea |
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FANG Yan1,2, PAN Li-xia1, YI Xiang-xi3, LIN Lin1, WANG Yi-bing1, GAO Cheng-hai1
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| (1.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi University, Nanning, Guangxi, 530004, China;3.Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, 530001, China) |
| Abstract: |
| Based on partial morphological analysis, 90 bacterial strains were isolated from the Gorgonian Anthogorgia caerulea obtained from Weizhou island, Guangxi, by means of maine nutrient agar, marine agar 2216, improved LB sea agar and sea water agar.Among them, 11 bacterial strains had antifouling activities against four fouling bacteria, furthermore, 5 bacterial strains effectively inhibited larval settlement of B.neritina and B.amphitrite larvae.Five strains were selected to perform a phylogenetic analysis based on 16S rRNA gene sequences, and the results showed that these 5 isolates belong to Bacillus genus (Bacillus sp.). |
| Key words: Gorgonian Anthogorgia caerulea marine bacterial strains antifouling |
