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  • 李检秀,孙靓,陈东,郭铃,黄艳燕,孙菲菲.一株耐酸L-乳酸生产菌的Co60诱变选育[J].广西科学,2011,18(4):392-395.    [点击复制]
  • LI Jian-xiu,SUN Liang,CHEN Dong,GUO Ling,HUANG Yan-yan,SUN Fei-fei.Screening of an Acid Tolerant Lactobacillus acidophilus strain by Co60 Mutagenesis[J].Guangxi Sciences,2011,18(4):392-395.   [点击复制]
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一株耐酸L-乳酸生产菌的Co60诱变选育
李检秀, 孙靓, 陈东, 郭铃, 黄艳燕, 孙菲菲
0
(1. 广西科学院非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物炼制重点实验室, 广西南宁 530007)
摘要:
对嗜酸乳杆菌(Lactobacillus acidophilus) CICC 6005菌株进行Co60诱变,从突变体中选育得到1株生长快速的耐酸突变菌株LS-8。该菌株在pH值3.6的条件下发酵72h、在pH值3.8的条件下发酵104h,L-乳酸产量分别达9.2g/L和16.5g/L。
关键词:  嗜酸乳杆菌  Co60诱变  L-乳酸酸性发酵
DOI:
投稿时间:2011-08-09
基金项目:广西科学院基本科研业务费项目(10YJ25SW11)资助
Screening of an Acid Tolerant Lactobacillus acidophilus strain by Co60 Mutagenesis
LI Jian-xiu, SUN Liang, CHEN Dong, GUO Ling, HUANG Yan-yan, SUN Fei-fei
(State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China)
Abstract:
Originally from Lactobacillus acidophilus CICC 6005, an acid tolerance mutant strain LS-8 was obtained by Co60 mutagenesis and the selected strain had high growing speed as target.At pH value 3.6 and 3.8, the yield of L-lactic acid by fermentation of LS-8 for 72h and 104h were 9.2g/L and 16.5g/L, respectively, which revealed much higher than that of reported investigation.
Key words:  Lactobacillus acidophilus  Co60 mutagenesis  L-lactic acid fermentation

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