| 引用本文： |
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潘诗翰,陈姗姗,黄庶冰,龙明华,黄庶识.毕赤酵母发酵甘露醇产乙醇的条件研究[J].广西科学,2011,18(2):164-168. [点击复制]
- PAN Shi-han,CHEN Shan-shan,HUANG Shu-bing,LONG Ming-hua,HUANG Shu-shi.Study on Conditions of Ethanol Fermentation of Mannitol by Pichia angophorae[J].Guangxi Sciences,2011,18(2):164-168. [点击复制]
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| 摘要: |
| 在250ml三角瓶中进行毕赤酵母(Pichia angophorae)发酵不同浓度的甘露醇生产乙醇的试验。试验先以浓度为20g/L、40g/L、60g/L、80g/L的甘露醇进行发酵试验确定出最适培养基组分,然后以正交试验确定菌株的最优发酵条件。结果确定出最适的发酵培养基组分为:甘露醇20g/L,酵母浸粉0.3g/L,麦芽浸粉0.3g/L,(NH4)2SO4 5g/L,KH2PO4 2g/L,MgSO4·7H2O 0.4g/L。最佳发酵条件为:温度32℃,摇床转速150rpm,初始pH值4.5,发酵液体积150ml,乙醇最大产量为0.45g ethanol/g mannitol。 |
| 关键词: 毕赤酵母 甘露醇 发酵 乙醇 |
| DOI： |
| 投稿时间：2011-02-24修订日期：2011-03-30 |
| 基金项目:广西自然科学基金重点项目(2010GXNSFD013029);广西科学院基本科研业务费项目(10YJ25WL01);人力资源和社会保障部留学人员科技活动项目择优经费项目(人社厅函[2010]412号)资助 |
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| Study on Conditions of Ethanol Fermentation of Mannitol by Pichia angophorae |
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PAN Shi-han1,2, CHEN Shan-shan1,3, HUANG Shu-bing2, LONG Ming-hua2, HUANG Shu-shi1
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| (1.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi University, Nanning, Guangxi, 530004, China;3.The College of life and Science, Guangxi Normal University, Guilin, Guangxi, 541004, China) |
| Abstract: |
| Ethanol fermentation of different concentration of mannitol by Pichia angophorae was carried out in 250ml Erlenmeyer flasks.The optimum composition of fermentation medium was determined by the tests with concentration of 20g/L, 40g/L, 60g/L and 80g/L of mannitol.Then an orthogonal test was designed and performed, searching for the best fermentation condition.The results indicated that the optimum medium composition for ethanol fermentation was 20g/L mannitol, 0.3g/L yeast extract, 0.3g/L malt extract, 5g/L (NH4)2SO4, 2g/L KH2PO4 and 0.4g/L MgSO4·7H2O.Besides, the optimal fermentation condition was 32℃, 150rpm, initial pH value 4.5 and 150ml fermentation liquor with a yield of 0.45 g ethanol (g mannitol)-1. |
| Key words: Pichia angophorae mannitol fermentation ethanol |
