| 引用本文： |
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张搏,王青艳.导入脂肪酶基因提高荧光假单胞菌26-2的产酶效率[J].广西科学,2009,16(2):185-187. [点击复制]
- ZHANG Bo,WANG Qing-yan.Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain[J].Guangxi Sciences,2009,16(2):185-187. [点击复制]
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| 摘要: |
| 采用导入脂肪酶基因的方法对荧光假单胞菌(Pseudomonas fluorescence)26-2的产酶效率进行实验。实验以质粒ppic9k-lipA为模板,通过PCR的方式在26-2脂肪酶基因的两端引入Bam H1和EcoR1的酶切位点,并将该基因与大肠杆菌荧光假单胞菌穿梭质粒pDSK519连接,获得重组质粒pDSK519-lipA,再将重组质粒转入荧光假单胞菌26-2,获得工程菌株P.fluorescence 26-2-1。在相同的发酵条件下,工程菌株的发酵液酶活力比原始菌株的发酵液酶活力提高2倍,实现了荧光假单胞菌脂肪酶基因的同源性表达。 |
| 关键词: 荧光假单胞菌 同源性表达 脂肪酶基因 |
| DOI： |
| 投稿时间：2008-11-17 |
| 基金项目:广西科学院创新基金项目(NO.桂科院研0701)资助 |
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| Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain |
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ZHANG Bo, WANG Qing-yan
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| (Guangxi Academy of Science, Nanning, Guangxi, 530007, China) |
| Abstract: |
| A Pseudomonas fluorescence lipase gene lipA was subcloned from ppic9k-lipA and inserted into vector pDSK519 to construct a new plasmid,pDSK519-lipA,which was transformed into its original strain P.fluorescence 26-2.The recombinant strain 26-2-1 was confirmed by restriction digestion.Compared with wild type 26-2 in pot experiment and ferment experiment,the lipase production of 26-2-1 is 2 times more than its original strain. |
| Key words: P.fluorescence homologous expression lipase gene |
