| 引用本文： |
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王卓渊,沈星灿,闫海刚,梁宏.Zn(Ⅱ)、Mn(Ⅱ)竞争结合人血清蛋白研究[J].广西科学,2009,16(2):170-173. [点击复制]
- WANG Zhuo-yuan,SHEN Xing-can,YAN Hai-gang,LIANG Hong.Studies of Competitive Binding for Human Serum Albumin between Zn (Ⅱ) and Mn (Ⅱ)[J].Guangxi Sciences,2009,16(2):170-173. [点击复制]
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| 摘要: |
| 用平衡透析法在生理pH值(7.43)条件下,研究了等摩尔比的Zn(Ⅱ)与Mn(Ⅱ)离子竞争结合人血清白蛋白(HSA)。Scatchard图分析表明,Zn(Ⅱ)与Mn(Ⅱ)在HSA中没有共同的强结合位点,在竞争和非竞争结合时,Zn(Ⅱ)的强结合位点数目均为1,Mn(Ⅱ)的强结合位点数目均为2。Hill图分析说明Zn(Ⅱ)与Mn(Ⅱ)之间具有一定的正协同效应。 |
| 关键词: 锌(Ⅱ) 锰(Ⅱ) 血清白蛋白 竞争结合 协同效应 |
| DOI： |
| 投稿时间：2008-11-17 |
| 基金项目:国家自然科学基金项目(No.29961001);广西壮族自治区教育厅项目(No.桂教科研[1998]334);广西"十百千人才工程"项目(No.桂政发[1998]60)资助 |
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| Studies of Competitive Binding for Human Serum Albumin between Zn (Ⅱ) and Mn (Ⅱ) |
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WANG Zhuo-yuan, SHEN Xing-can, YAN Hai-gang, LIANG Hong
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| (Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education, Guangxi Normal University, Guilin, Guangxi, 541004, China) |
| Abstract: |
| Equilibrium dialysis was used to investigate the competition binding for human serum albumin (HSA) between equimolar Zn (Ⅱ) and Mn (Ⅱ) ions at physiological pH (7.43).The results of Scatchard plots indicated that HSA possesses two strong binding sites for Mn (Ⅱ),and one strong binding site for Zn (Ⅱ).The competitive bindings have no insignificant effect on the number of strong binding sites for Zn (Ⅱ) and Mn (Ⅱ),which suggested that there are not any common strong binding sites for Zn (Ⅱ) and Mn (Ⅱ) in HSA.Whereas,the competitive binding increased the number of weak binding sites of Zn (Ⅱ) and Mn (Ⅱ) in serum albumins.The analyses of Hill plots also showed that the cooperative effect existed between Zn (Ⅱ) and Mn (Ⅱ) ions during the process of competitive binding with HSA. |
| Key words: Zinc (Ⅱ) Manganese (Ⅱ) serum albumin competitive binding cooperative effect |
