| 引用本文： |
-
付传明,黄宁珍,赵志国,唐凤鸾,李锋.广西地不容种质离体保存技术研究[J].广西科学,2007,14(2):155-159. [点击复制]
- FU Chuan-ming,HUANG Ning-zhen,ZHAO Zhi-guo,TANG Feng-luan,LI Feng.Study on Preservation of Stephanie kwangsiensis H.S.Lo in Vitro[J].Guangxi Sciences,2007,14(2):155-159. [点击复制]
|
|
| 摘要: |
| 以广西地不容(Stephanie kwangsiensis H.S.Lo))试管苗为材料,研究室温(25±2)℃光照2000lx(12h/d)条件下,培养基中不同无机盐水平(MS、1/2MS、1/4MS)、蔗糖浓度(0、20、40、60g/L)和植物生长抑制剂CCC(0.4、0.8、1.2、1.6、2.0、2.5mg/L)、PP333(0.2、0.5、1.0、2.0、4.0、8.0mg/L)、ABA(0.2、0.5、1.0、2.0、4.0、8.0mg/L)、MH(0.2、0.5、1.0、2.0、4.0、8.0mg/L)对广西地不容试管苗保存存活率的影响。结果表明,MS添加蔗糖20~40g/L适合作为广西地不容离体保存的基本培养基,附加一定浓度的CCC、PP333、ABA和MH能有效抑制试管苗的旺盛生长,长时间保存后的存活率高;ABA易引起试管苗早衰,保存效果不佳。对广西地不容试管苗保存效果较好的两个培养基配方为:MS+CCC0.4~2.5mg/L+蔗糖30g/L和MS+PP3330.2~0.5mg/L+蔗糖30g/L,这两个配方可以不继代连续保存360~380d,存活率60%~80%。 |
| 关键词: 广西地不容 离体保存 生长抑制剂 矮壮素 多效唑 脱落酸 青鲜素 |
| DOI： |
| 投稿时间：2007-01-17修订日期：2007-03-20 |
| 基金项目:广西科技攻关(桂科攻0322024-3B)项目资助 |
|
| Study on Preservation of Stephanie kwangsiensis H.S.Lo in Vitro |
|
FU Chuan-ming, HUANG Ning-zhen, ZHAO Zhi-guo, TANG Feng-luan, LI Feng
|
| (Guangxi Institute of Botany, Guilin, Guangxi, 541006, China) |
| Abstract: |
| The effects of media (MS、1/2MS and 1/4MS), concentration of sucrose and plant retardants (CCC、PP333、ABA、MH)on preservation of Stephanie kwangsiensis H.S.Lo in vitro were studied. According to the results, MS medium with sucrose 20~40g/L could be used as basic medium for the preservation of Stephanie kwangsiensis H.S.Lo in vitro.CCC、PP333 and MH in certain concentration could retard the growth of tube plantlets which could be preserved for a long time with high survival rate.But tube plantlets tended to senesce prematurely in the mediums with ABA, in which effects on preservation were not very satisfied.The results showed that MS+CCC 0.4~2.5mg/L+sucrose30g/L and MS+PP333 0.2~0.6mg/L+sucrose 30g/L were better than other combinations in preservation of Stephanie kwangsiensis H.S.Lo in vitro.These two formulas could store the tube plantlets up to the (without transfer) 360~380d at 60%~80% survival rate. |
| Key words: Stephanie kwangsiensis H.S.Lo preservation in vitro plant growth retardant CCC PP333 ABA MH |
