| 引用本文： |
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秦新民,梁燕妮,黄夕洋.3个地理种群蛤蚧不同组织同工酶分析[J].广西科学,2006,13(4):310-315. [点击复制]
- QIN Xin-min,LIANG Yan-ni,HUANG Xi-yang.Isozymes Analysis on Different Tissues from Three Different Populations of Gekko gecko[J].Guangxi Sciences,2006,13(4):310-315. [点击复制]
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| 摘要: |
| 采用垂直板状聚丙烯酰胺凝胶电泳(PAGE)方法,比较泰国、广西和越南地理蛤蚧(Gekko gecko)种群的肌肉、心、肝、肺、胃、肠、性腺、肾等8种组织的乳酸脱氢酶(LDH)、酯酶(EST)和乙醇酸脱氢酶(ADH)同工酶。结果表明:广西种群和越南种群的LDH同工酶谱带在肌肉、心脏、肝脏、肺、胃和肾组织中完全相同,泰国种群各种组织中的LDH同工酶与广西和越南种群相比差异较大,主要表现在有较多的亚带,三个不同地理种群的8种组织几乎均表现出明显的5条LDH同工酶主带,但不同种群不同组织的谱带着色深浅不同;各组织EST同工酶谱无论从带型,谱带着色深浅来看,泰国种群均与其它两个种群差别较大;三个种群8种组织ADH同工酶的带型基本相同,迁移率也大致相同,但谱带着色深浅不同。 |
| 关键词: 蛤蚧 同工酶 乳酸脱氢酶 酯酶 乙醇酸脱氢酶 |
| DOI： |
| 投稿时间：2006-04-28修订日期：2006-06-22 |
| 基金项目:广西博士学位授权学位点学科建设和广西教育厅项目联合资助 |
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| Isozymes Analysis on Different Tissues from Three Different Populations of Gekko gecko |
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QIN Xin-min, LIANG Yan-ni, HUANG Xi-yang
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| (College of Life Science, Guangxi Normal University, Guilin, Guangxi, 541004, China) |
| Abstract: |
| By using the vertical polyacrylamide gel electrophoresis,three isozymes,LDH、EST and ADH from muscle,heart,liver,lungs,stomach,intestinum,sex gland and kidney of Gekko gecko collected from Thailand,Guangxi China and Viet Nam were studied.The results showed that the three different geographical populations and eight tissues had different and distinct isozyme patterns. |
| Key words: Gekko gecko isozyme LDH EST ADH |
