| 引用本文： |
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覃兰芳,赖茂祥,梁威.RP-HPLC测定中风康复片中士的宁含量[J].广西科学,2006,13(3):219-221. [点击复制]
- QIN Lan-fang,LAI Mao-xiang,LIANG Wei.Determination of Strychnine in Zhongfeng Kangfu Tabletby by RP-HPLC[J].Guangxi Sciences,2006,13(3):219-221. [点击复制]
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| 摘要: |
| 采用反相高效液相色谱法(RP-HPLC),以Kromasil C18柱(250×4.6mm,5μm)为色谱柱,甲醇-0.05M磷酸二氢钾(25:75)为流动相,在254nm检测波长,1.0m l/min流速的条件下建立中风康复片中士的宁的含量测定方法。结果表明,中风康复片中士的宁与其它成分分离良好,士的宁在0.072~0.648μg范围内呈良好的线性关系(r=0.9999),测量方法的平均回收率为95.51%,RSD=0.7%(n=6)。该方法灵敏度高、操作简单、结果准确,可用于本制剂的质量控制。 |
| 关键词: 中风康复片 士的宁 反相高效液相色谱法 |
| DOI： |
| 投稿时间：2005-12-22修订日期：2006-03-15 |
| 基金项目: |
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| Determination of Strychnine in Zhongfeng Kangfu Tabletby by RP-HPLC |
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QIN Lan-fang1, LAI Mao-xiang2, LIANG Wei1
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| (1.Guangxi Research Center of Natural Medicine, Nanning, Guangxi, 530022, China;2.Guangxi Institute of Traditional Medical and Pharmaceutical Science, Nanning, Guangxi, 530022, China) |
| Abstract: |
| To establish a RP-HPLC method for determination of strychnine in Zhongfeng Kangfu Tablet.The sample were analyzed by RP-HPLC using Kromasil (250×4.6mm, 5μm) column;methanol -0.05M potassium dihydrogen phosphate (25:75) as mobile phase;UV detector at 245nm and the flow rate was at 1.0ml/min.The result was the content of strychnine had a good linearity in the range of 0.072~0.648 (μg), the correlation was 0.9999, the Average recovery was 95.51%, RSD=0.7% (n=6).The method was sensitive, simple, accurate and suitable for quality control of strychnine in Zhongfeng Kangfu tablet. |
| Key words: Zhongfeng Kangfu tablet strychnine RP-HPLC |
