| 引用本文： |
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蔡爱华,何成新,曾健智,张厚瑞,徐庆.用于生产低聚木糖的木聚糖酶菌株筛选[J].广西科学,2004,11(4):339-342. [点击复制]
- Cai Aihua,He Chengxin,Zeng Jianzhi,Zhang Hourui,Xu Qing.A Screening of Xylanase Producing Strains for Xylooligosaccaride Production[J].Guangxi Sciences,2004,11(4):339-342. [点击复制]
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| 摘要: |
| 比较分别属于木霉属(Trichoderma)、毛壳属(Chaetomium)、黑曲霉属(Aspergillus)、芽孢杆菌属(Bacillus)的12个菌株的木聚糖酶活性,以蔗渣木聚糖为底物,分析不同菌株木聚糖酶水解产物中的糖类组成,考察木聚糖酶系的稳定性。结果表明,毛壳属菌株所产木聚糖酶的水解产物中,低聚木糖的比例普遍高于其它类型菌株,其中球毛壳AS3.3601不仅木聚糖酶的活性较高,而且酶系稳定性最好,水解液中木二糖、木三糖占总糖含量的76%以上。球毛壳AS3.3601的发酵液按最大剂量0.4ml/10g给小鼠灌胃,每天1次,连续7d,所有受试小鼠均无中毒反应,初步认为球毛壳AS3.3601对于口服是安全的。 |
| 关键词: 低聚木糖 木聚糖酶 菌株 筛选 |
| DOI： |
| 投稿时间：2004-09-13 |
| 基金项目:广西青年科学基金(NO.0007010)与广西创新能力建设基金(No.033015-1B)资助项目。 |
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| A Screening of Xylanase Producing Strains for Xylooligosaccaride Production |
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Cai Aihua1, He Chengxin1, Zeng Jianzhi1, Zhang Hourui1, Xu Qing2
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| (1.Guangxi Institute of Botany, Yanshan, Guilin, Guangxi, 541006, China;2.The Pharmic Dept. of Guilin Medicine College, Lequnlu, Guilin, Guangxi, 541002, China) |
| Abstract: |
| The xylanase activity of 12 different strains belonging to Trichoderma,Chaetomium,Aspergillus and Bacillus were contrasted.Using the xylan of sugar cane bagasse as subtracts,the carbohydrate components in the hydrolysis solution of xylanases from different strains were analysed and the steadity of xylanases from some strains were also evaluated.The results showed that the enzymatic products by the xylanase from the strains of Chaetomium contained commonly much more xyloolysaccharides than those from the others.The best performance happened in Chaetomium globosum AS3.3601,which produced both the high activity xylanase and good steadity of enzymatic systems,further more,the amount of xylobiose and xylotriose occupied at least 76 percent of the total sugar in its enzymatic products.Force feeding the mice with broth of Chaetomium globosum AS3.3601 once per day at the maximum amount of 0.4ml/10g lasted for 7 days.At the end,none of the treated mice was poisoned.Therefore we can tentatively regard Chaetomium globosum AS3.3601 as safe strain for orally taking. |
| Key words: xylooligosaccharide xylanase strain screening |
