| 引用本文： |
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崔建国,黄燕敏,何小玉,曾陇梅.多羟基甾醇的合成(Ⅳ):20-亚甲基-4-孕烯-3β,6β-二醇的制备及其抗肿瘤活性研究[J].广西科学,2004,11(1):43-46. [点击复制]
- Cui Jianguo,Huang Yanmin,He Xiaoyu,Zeng Longmei.Synthesis of Polyhydroxysterols(Ⅳ): The Synthesis of 20-methylene-4-pregnene-3β,6β -diol,and theInvestigation of Its Anticancer Activity[J].Guangxi Sciences,2004,11(1):43-46. [点击复制]
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| 摘要: |
| 从孕烯醇酮(4)出发,经过6步反应合成新的多羟基甾醇化合物20-亚甲基-4-孕烯-3β,6β-二醇(3),总收率为33.40%,并通过IR、1H NMR等谱图数据对化合物(3)的结构进行表征。用0.005mg/ml化合物(3)和0.05mg/ml的4-孕烯-3β,6β-二羟基-20-酮(9),通过MTT法对宫颈癌细胞(HELA)及白血病细胞(P-388)进行抗肿瘤细胞活性研究,结果表明,化合物(3)对HELA和P-388的生长均没有产生明显的细胞毒性。 |
| 关键词: 甾醇 20-亚甲基-4-孕烯-3β 6β-二醇 结构表征 抗肿瘤活性 |
| DOI： |
| 投稿时间：2003-10-16 |
| 基金项目:国家自然科学基金资助项目(批准号:29932030)。 |
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| Synthesis of Polyhydroxysterols(Ⅳ): The Synthesis of 20-methylene-4-pregnene-3β,6β -diol,and theInvestigation of Its Anticancer Activity |
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Cui Jianguo1, Huang Yanmin1, He Xiaoyu1, Zeng Longmei2
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| (1.Chemistry Department, Guangxi Teachers College, East Mingxiulu, Nanning, Guangxi, 530001, China;2.Coll. of Chemistry & Chemieal Engi., Sun Yat-Sen University, Guangzhou, Guangdong, 510275, China) |
| Abstract: |
| Using pregnenolone (4) as a starting material,20-methylene-4-pregnene-3β,6β -diol (3) was synthesized in 6 steps in an overall yield of 33.40%.The structure of (3) is defermined by IR and 1H NMR analysis.The study of cytotoxicity of 2 against cancer cells showed that 2 is inactive against the growth of human cervical carcinoma cells (HELA) and leukemia cells (P-388). |
| Key words: sterols 20-methylene-4-pregnene-3β 6β-diol structure elucidation anticancer activity |
