| 引用本文： |
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苏建家,李瑗,王树声,班克臣,覃柳亮,欧超,张华.树鼩对人乙型肝炎病毒易感性的验证[J].广西科学,2000,7(4):298-301. [点击复制]
- Su Jianjia,Li Yuan,Wang Shusheng,Ban Kechen,Qin Liuliang,Ou Chao,Zhang Huayuan.Susceptibility Inspecting for the Human Hepatitis B Virus Infection in Tree Shrews[J].Guangxi Sciences,2000,7(4):298-301. [点击复制]
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| 摘要: |
| 为验证树鼩对人乙型肝炎病毒(HBV)的易感性,用含HBV的人血清接种给10只成年树鼩(雌雄各半)。然后每周抽血1次,每只动物共抽血11次。用不同公司生产的ELISA试剂检测接种后的动物血清感染指标。实验观察至13周,结果分别有9只和7只动物出现HBsAg阳性,持续最短1周,最长7周。用PCR检测,结果有1只动物连续4周在血清中检测出HBVDNA。表明树鼩能感染人HBV,但实验有待改进以延长感染持续时间。 |
| 关键词: 树鼩 乙型肝炎病毒 感染 |
| DOI： |
| 投稿时间：2000-07-04 |
| 基金项目:国家"九五"攻关项目"乙型肝炎动物模型研究"(96-906-03-10)。 |
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| Susceptibility Inspecting for the Human Hepatitis B Virus Infection in Tree Shrews |
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Su Jianjia, Li Yuan, Wang Shusheng, Ban Kechen, Qin Liuliang, Ou Chao, Zhang Huayuan
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| (Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China) |
| Abstract: |
| To examine the tree shrew's susceptibility for human hepatitis B virus,ten adult tree shrews (5 males,5 females) were inoculated by human sera with HBV. After that, weekly blood sampling was carried out one time each animal lasted for 11 weeks. HBsAg was detected with ELISA kits purchased from two different companies. Results showed that 9 and 7 animals in this experiment were positive for HBsAg respectively. One animal was found HBV DNA in sera by PCR, which lasted for 4 weeks. The data indicates that tree shrew could be infected with human HBV, but the methods of inoculation or conditions need to be improved to achieve a chronic HBV infection with more persistence of viral replication. |
| Key words: tree shrew hepatitis B virus infection |
