| 引用本文： |
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孙文波,李海鹰,王桂文,范嘉晔.应用RAPD鉴定红菇组织分离菌株的探索试验[J].广西科学,2000,7(3):222-224. [点击复制]
- Sun Wenbo,Li Haiying,Wang Guiwen,Fan Jiaye.Identification of Russula's Isolates with RAPD[J].Guangxi Sciences,2000,7(3):222-224. [点击复制]
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| 摘要: |
| 用组织分离技术从野生红菇(Russula sp.)子实体中分离得3个菌株。用随机扩增多态性DNA(RAPD)对红菇子实体和分离菌株的DNA多样性进行分析,以确定3个分离菌株与子实体之间的亲缘关系。实验使用16个随机引物检测40多个位点,计算它们之间的相似性。实验结果表明3个分离菌株和红菇子实体之间的DNA相似性非常高,红菇子实体和对照菌株[凤尾菇(Pleurotus sajor-caju)和金针菇(Flammulina velutipes(Fr) Sing)]之间的DNA相似性差距基本上反映了红菇与对照菌株之间的种属差距。推测3个红菇分离菌株同一个种。 |
| 关键词: 红菇 分离菌株 亲缘关系 随机扩增多态性DNA |
| DOI： |
| 投稿时间：1999-07-29修订日期：2000-03-02 |
| 基金项目:国家自然科学基金资助项目(3945005)、广西自然科学基金资助项目(桂科配9618005)。 |
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| Identification of Russula's Isolates with RAPD |
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Sun Wenbo, Li Haiying, Wang Guiwen, Fan Jiaye
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| (Institute of Biology, Guangxi Academy of Sciences, 2 Dalinglu, Nanning, Guangxi, 530003, China) |
| Abstract: |
| Three strains were obtained from the sporocarp of wild Russula using tissue culture isolation.Random amplified polymorphic DNA(RAPD) was used to approach genetic diversity of wild Russula and three strains, so as to decide the relation between three strains and sporocarp. Over forty genetic sites were checked by using sixteen random primers, and the genetic similarity between three strains and Russula sporecarp was studied.The result showed that they were greatly similar and the similarity gap of DNA between Russula sporocarp and the checked strains showed the interspecies distance. A preliminary conclusion can be drawn from the experiment that the three strains were isolates of Russula. |
| Key words: Russula strain relative random amplified polymorphic DNA |
