| 引用本文： |
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周兴,李海鹰,王桂文,范嘉晔.花叶开唇兰的初级培养及再生途径[J].广西科学,1999,6(2):157-159. [点击复制]
- Zhou Xin,Li Haiying,Wang Guiwen,Fan Jiaye.Primary Culture and Regeneration Ways of Anoectochilus roxburghii[J].Guangxi Sciences,1999,6(2):157-159. [点击复制]
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| 摘要: |
| 结果表明:培养基的组成对初级培养成活率影响不大,死亡率高、萌发率低是由于花叶开唇兰肉质茎脆弱,抗消毒剂能力差。要提高初级培养成活率应尽量不破坏叶鞘,控制好消毒强度,采种时间以1月为宜。芽的再生途径:一是节间腋芽萌发形成主芽,增殖系数2~2.5;二是原球茎或丛生芽,增殖系数3~4。前一种再生途径形成的芽对外界气温适应能力较强,后一种适应性较差,28℃以上气温,生长受抑制甚至出现大量死亡现象。试验材料为广西那坡野生花叶开唇兰,植株6节高、茎径大于0.6cm。 |
| 关键词: 花叶开唇兰 初级培养 再生途径 |
| DOI： |
| 投稿时间：1998-04-28修订日期：1998-07-08 |
| 基金项目:广西科学院基金资助项目(1995年)。 |
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| Primary Culture and Regeneration Ways of Anoectochilus roxburghii |
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Zhou Xin, Li Haiying, Wang Guiwen, Fan Jiaye
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| (Institute of Biology, Guangxi Academy of Sciences, 2 Dalinglu, Nanning, Guangxi, 530003) |
| Abstract: |
| Experiments showed that the ingredients of medium had a little effect on the survival rate; the high mortality and low sprouting rate were due to the weak pulped stem and low resistant to sterilizing.For raising its survival rate, leaf sheeth should be well kepted on the stem and the sterilizing intensity should be in a good control, collecting seedlings in January. The plants are regenerated from both the axillary buds with a multiplication coefficient 2~2.5,and the protocorm with a multiplication coefficient 3~4.The buds from the former way had a good adaption to the outside temperature, while those from the latter way are poor in adaptation and may die at above 28℃.The trial materials were wild Anoectochilus roxburghii collected from Napo county of Guangxi, and plants were 6 nodes high with stem greater than 0.6 cm in diameter. |
| Key words: Anoectochilus roxburghii primary culture ways of regeneration |
