| 引用本文： |
-
姚德生,李力,赵仁峰,张洁清,唐步坚,古明华,陈心秋.CD44V3在恶性卵巢肿瘤中的表达及其临床意义[J].广西科学,1999,6(2):124-126. [点击复制]
- Yao Desheng,Li Li,Zhao Renfeng,Zhang Jieqing,Tang Bujian,Gu Minghua,Chen Xinqiu.Expression of CD44V3 and Its Clinical Significance in Malignant Ovarian Tumor[J].Guangxi Sciences,1999,6(2):124-126. [点击复制]
|
|
| 摘要: |
| 为探讨卵巢恶性肿瘤组织中CD44V3的表达及其临床意义,用免疫组织化学和RT-PCR方法,检测41例恶性卵巢肿瘤、20例良性卵巢肿瘤和21例正常卵巢组织中CD44V3基因及基因蛋白的表达情况,并分析相关的临床病理因素。结果:CD44V3表达阳性率恶性组显著高于良性组和正常组(均P<0.05);有转移者CD44V3阳性率显著高于无转移者(P<0.01),转移灶阳性率高于原发灶(P=0.01);CD44V3阳性表达者近期疗效比阴性者差(P<0.05);CD44V3阳性表达率与临床期别有一定关系,但与病理类型、组织学级别无关(P>0.05)。表明,CD44V3与肿瘤的发生、发展、卵巢恶性肿瘤的浸润和转移、以及近期疗效有关,可以作为预测肿瘤转移潜能和评估预后的指标。 |
| 关键词: CD44 PCR 卵巢肿瘤 免疫组织化学 肿瘤转移 |
| DOI： |
| 投稿时间：1998-07-17修订日期：1998-11-09 |
| 基金项目:国家自然科学基金资助(编号39560081)。 |
|
| Expression of CD44V3 and Its Clinical Significance in Malignant Ovarian Tumor |
|
Yao Desheng, Li Li, Zhao Renfeng, Zhang Jieqing, Tang Bujian, Gu Minghua, Chen Xinqiu
|
| (Cancer Hospital, Guangxi Medical University, 6 Binhulu, Nanning, Guangxi, 530021) |
| Abstract: |
| To detect the expression of CD44V3 and to investigate its clinical significance in malignant ovarian tumor,the reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemical stain(IHCS) were used to detect the mRNAs content and expression proteins of CD44V3 in biopsy samples of 41 malignant ovarian tumors, 20 benign ovarian tumors and 21 normal controls.In the positive expression rates of CD44V3(PERCD),the malignance was higher than the benign and control(all P<0.05);the residual focus of >2 cm was higher than the residual focus of ≤ 2 cm (P<0.01);the metastatic focus was higher than primary focus (P=0.01);the primary focus with metastasis were higher than that without metastasis (P<0.01).The short term curative effects of the patient with positive expression of CD44V3 were lower than that of the negative patients (P<0.05).No significant correlation was observed between PERCD and pathological type, or histological grade in cancer tissue.It suggests that CD44V3 correlate with the carcinogenesis and progress of tumor, the metastasis of malignant ovarian tumor and the curative effects;CD44V3 may be used for predicting the metastasis of cancer and would be a new marker for evaluating the curative effects and prognosis. |
| Key words: CD44 PCR ovarian neoplasms Immunohistochemistry metastasis |
