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  • 涂楚桥,梁宏,王光辉.Cl-、NO3-和SO42-离子对漆酶催化活性的抑制作用[J].广西科学,1998,5(4):285-287,303.    [点击复制]
  • Tu Chuqiao,Liang Hong,Wang Guanghui.The Inhibition of Cl-、NO3- and SO42- Ions on Laccase-enzymatic Activity[J].Guangxi Sciences,1998,5(4):285-287,303.   [点击复制]
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Cl-、NO3-和SO42-离子对漆酶催化活性的抑制作用
涂楚桥, 梁宏, 王光辉
0
(广西师范大学化学化工系, 桂林市育才路3号 541004)
摘要:
以5,6-二溴-2,3-二氰基氢醌为底物,在pH值4.50条件下,用分光光度法考察了Cl-、NO3-和SO42-离子对漆酶催化活性的影响。发现Cl-、NO3-和SO42-离子对漆酶的催化活性均有不同程度的抑制作用,其最大抑制率分别可达75%、31.4%和17.1%。动力学研究表明,Cl-、NO3-和SO42-离子的抑制作用是瞬时完成的,属于竞争性抑制过程,是通过与底物分子竞争漆酶分子中的Ⅱ型铜(Ⅱ)部位实现的。经测定,Cl-和NO3-离子与漆酶所形成的复合物其解离常数分别为1.12×10-4 mol·dm-3和1.78×10-3 mol·dm-3
关键词:  漆树漆酶  阴离子  竞争性抑制作用  分光光度法
DOI:
投稿时间:1998-06-25
基金项目:广西自然科学基金资助课题。
The Inhibition of Cl-、NO3- and SO42- Ions on Laccase-enzymatic Activity
Tu Chuqiao, Liang Hong, Wang Guanghui
(Dept. of Chemistry, Guangxi Normal University, 3 Yucailu, Guilin, Guangxi, 541004)
Abstract:
The influence of Cl-、NO3- and SO42- ions on laccase-enzymatic activity was studied spectrophotometrically by 5,6-dibromo-2,3-dicyanohydroquinone (DDBQH2) under condition of pH4.50 and 30±0.1℃.The results showed that Cl-、NO3- and SO42- ions had obvious inhibition,and the inhibition ratio of theirs reached to 75%,31.4% and 17.1%,respectively;The kinetic studies demonstrated that the inhibitory actions of Cl-、NO3- and SO42- ions were time independent competitive type,and the inhibited position was the type 2 Cu(Ⅱ) site of laccase.The dissociation constants (Ki) of complexing reaction of Cl- and NO3- ions with type 2 Cu(Ⅱ) site of laccase are 112 and 1780 μmol·dm-3,respectively.
Key words:  Rhus vernicifera laccase  anions  competitive inhibition  spectrophotometry

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