| 引用本文： |
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罗元,苏建家,覃思繁,梁秀就.突变型P53蛋白、C-erbB-2癌蛋白和PCNA在大肠上皮性肿瘤中的表达[J].广西科学,1998,5(2):94-97. [点击复制]
- Luo Yuan,Su Jianjia,Qin Sifan,Liang Xiujiu.Expression of P53 Protein、C-erbB-2 Oncoprotein and Proliferating Cell Nuclear Antigen in Colorectal Tumours[J].Guangxi Sciences,1998,5(2):94-97. [点击复制]
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| 摘要: |
| 用免疫组化方法检测突变型P53蛋白、C-erbB-2癌基因蛋白和增殖细胞核抗原(PCNA)在60例大肠腺癌、29例腺瘤及33例息肉石蜡切片中的表达情况。结果P53在大肠癌与腺瘤中均过表达,阳性率分别为70%和48.28%;而在息肉与正常粘膜中不表达。P53的表达还与大肠癌的分化程度和Duke's分期以及与腺瘤的大小、类型和不典型增生程度有关(P<0.05)。C-erbB-2仅在大肠癌中表达,阳性率65%。其表达与大肠癌的分化,淋巴结转移及Duke's分期等均无关(P>0.05)。PCNA100%表达,其所表示的细胞增殖指数与P53的表达在大肠癌与腺瘤中均呈正相关(分别为r=0.6887,P<0.01及r=0.8696,P<0.01)。结果表明P53的表达与大肠上皮性肿瘤的生物学行为有着非常密切的关系。检测P53与PCNA的表达水平将对大肠癌的早期诊治以及分析大肠癌的增殖活性和恶性度等方面均有重要意义。C-erbB-2是大肠癌的癌性标志,有可能作为鉴别诊断依据之一。 |
| 关键词: 大肠肿瘤 P53 C-erbB-2 PCNA 免疫组化 |
| DOI： |
| 投稿时间：1997-06-06 |
| 基金项目: |
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| Expression of P53 Protein、C-erbB-2 Oncoprotein and Proliferating Cell Nuclear Antigen in Colorectal Tumours |
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Luo Yuan, Su Jianjia, Qin Sifan, Liang Xiujiu
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| (Dept. of Pathology, Affiliated Tumour Hospital, Guangxi Medical University, 6 Binghulu, Nanning, Guangxi, 530021) |
| Abstract: |
| The expressions of P53 protein C-erbB-2 oncoprotein and proliferating cell nuclear antigen (PCNA) in 60 colorectal carcinomas (CC), 29 colorectal adenomas (CA) and 33 colorectal polypus (CP) were detected by immunohistochemistry.The positive rate for P53 was 70% in CC and 48.26% in CA;CP and normal mucosa were uniformly negative. Expression of P53 protein showed a significant association with differentiation and Duke's stage in CC(P<0.05), and association with the size, type and dysplasia in CA. (P<0.05 or P<0.01). C-erbB-2 expression was found only in CC. and its positive rate was 65%. Expression of C-erbB-2 had no correlation with differentiation. lymphatic metastasis and Duke's stage in CC(P>0.05). Expression of PCNA was found in all colorectal tumours and a significant correlation was found between PCNA index and P53 positive rate in CC(r=0.6887, P<0.01) and CA (r=0.8696, P<0.01). The results suggested that there were very closely relation between P53 and colorectal tumours behaviour. The expression level of P53 and PCNA index might be considerably important to analysis of proliferative activity and malignancy of CC, and C-erbB-2 is a CC marker and might be used for differential diagnosis. |
| Key words: colorectal tumour P53 C-erbB-2 PCNA immunohistochemistry |
