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  • 庞宗文,陈桂光,梁静娟.根霉2#(Rhizopussp.2#)产酶条件研究[J].广西科学,1997,4(3):235-237.    [点击复制]
  • Pang Zongwen,Chen Guiguang,Liang Jingjuan.The Study of the Produced Condition of Rhizopus sp. 2#[J].Guangxi Sciences,1997,4(3):235-237.   [点击复制]
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根霉2#(Rhizopussp.2#)产酶条件研究
庞宗文, 陈桂光, 梁静娟
0
(广西大学食品发酵工程研究所, 南宁市西乡塘路10号 530004)
摘要:
对影响根霉2#(Rhizopus sp.2)产生生淀粉分解酶的碳源、氮源、无机盐、温度、pH值等进行优化组合试验,摸索出了一个最佳的产酶条件,该条件为:木薯渣5%、花生麸0.75%、尿素0.1%、K2HPO40.1%、MgSO4 0.075%、pH值5.0、32℃摇瓶培养96h。使用该条件可使得发酵酶液的酶活达到7.75U/mL。
关键词:  生淀粉分解酶  根霉  产酶条件
DOI:
投稿时间:1996-12-30修订日期:1997-07-10
基金项目:广西壮族自治区教委资助项目。
The Study of the Produced Condition of Rhizopus sp. 2#
Pang Zongwen, Chen Guiguang, Liang Jingjuan
(The Food and Fermentation Engineering Institute of Guangxi University, 10 Xixiangtanglu, Nanning, Guangxi, 530004)
Abstract:
The effects of medium components such as carbon,nitrogen,inorganic salt and culture conditions such as pH,temperature on Rhizopus sp. 2# were investigated. The optimum formative condition of the raw starch decomposing enzyme was the medium containing 5% cassava residue, 0.75% peanut meal, 0.1% urea,0.1% K2HPO4,0.075% MgSO 4·7H2O and pH5.0. The raw starch decomposing enzyme activity of Rhizopus sp. 2 cultivated on this condition in 32℃ for 96h reached 7.75 U/mL.
Key words:  raw starch decomposing enzyme  Rhizopus sp.  produced condition

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