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刘自民,韦毅,麻秀珍,王文,陈永久,张亚平.白头叶猴线粒体ND4基因和D-环区的序列及其分类地位初探[J].广西科学,1997,4(1):64-65,71. [点击复制]
- Liu Zimin,Wei Yi,Ma Xiuzhen,Wang Wen,Chen Yongjiu,Zhang Yaping.Preliminary Studies on the Classification of Trachypithecus francoisi based on mitochondrial ND4 and D-Loop DNA[J].Guangxi Sciences,1997,4(1):64-65,71. [点击复制]
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| 白头叶猴线粒体ND4基因和D-环区的序列及其分类地位初探 |
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刘自民1, 韦毅1, 麻秀珍2, 王文3, 陈永久3, 张亚平3
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| (1.广西区林业厅野生动植物和, 自然保护区管理站, 南宁市七星路 530022;2.中华人民共和国南宁动物, 检疫所, 南宁 530001;3.中国科学院昆明动物研究所细胞与分子进化开放研究实验室, 云南昆明 650223) |
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| 摘要: |
| 用PCR(多聚酶链式反应)扩增方法测定白头叶猴、黑叶猴和菲氏叶猴各1只的线粒体DNAND4整个基因(1377bp)和D-环500多bp序列。黑叶猴与菲氏叶猴的ND4基因序列碱基差异数高达123,基于ND4基因的遗传距离值为8.9%;白头叶猴与黑叶猴ND4基因序列间碱基差异数为25,遗传距离值为1.8%。在D-环区域,白头叶猴与黑叶猴间的遗传距离为4.3%,而白头叶猴、黑叶猴两者与菲氏叶猴间的遗传距离则分别高达26.4%和26.0%。分析结果表明,白头叶猴恐怕还只是黑叶猴的一个亚种。从保护遗传学角度看,白头叶猴应被看作一个进化显著性单元(evolutionary significant units,ESU),在动物的保护行动中应得到充分的重视。 |
| 关键词: 白头叶猴 ND4序列 D-环序列 分类地位 |
| DOI： |
| 投稿时间：1996-12-19 |
| 基金项目:得到国家杰出青年人才基金、中科院"九五"重大项目支持。 |
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| Preliminary Studies on the Classification of Trachypithecus francoisi based on mitochondrial ND4 and D-Loop DNA |
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Liu Zimin1, Wei Yi1, Ma Xiuzhen2, Wang Wen3, Chen Yongjiu3, Zhang Yaping3
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| (1.The Wildlife and Nature Reserve Managing station of Guangxi Department of Forestry, Qixing Road, Nanning, Guangxi, 530022;2.Nanning Animal Quarantine Service of P. R. China, Nanning, 530001;3.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, 650223) |
| Abstract: |
| Mitochodrial ND4 (1377 bp) and D-Loop DNA (500 bp) sequences were employed for estimating phylogenetic relationships among white-head leaf monkey,black leaf monkey,and phayrei leaf monkey.The variable sites and genetic distance of ND4 sequences between black leaf monkey and phayrci leaf monkey were 123,and 8.9% respectively,while those between white-head leaf monkey and black leaf monkey were 25,and 1.8%.Mitochondrial D-Loop DNA was characteristic by evoluating faster.Based on D-Loop DNA analysis, the genetic distance between white-head leaf monkey and black leaf monkey was 4.3%,while those between white-head monkey and black leaf monkey were 26.4%,and 26.0% respectively.So far the black leaf monkey and phayrei leaf monkey have been valid species.Our studies implied that white-head leaf monkey should be a subspecies of black leaf monkey,but it should be a evolutionary significant units(ESU),which should be taken seriously in genetic conservation. |
| Key words: white-head leaf monkey ND4 sequence D-Loop sequence classification |
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