| 引用本文： |
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查冬兴,唐纪良,马庆生.转座子诱变甘蓝黑腐病菌所获胞外多糖突变体的类型[J].广西科学,1997,4(1):34-37. [点击复制]
- Zha Dongxing,Tang Jiliang,Ma Qingsheng.Classification of the Exopolysaccharide-deficient Mutants of Xanthomonas campestris pv. campestris Derived from Transposon Mutagenesis[J].Guangxi Sciences,1997,4(1):34-37. [点击复制]
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| 摘要: |
| 根据对转座子Tn5gusA5诱变甘蓝黑腐病菌所获得39株胞外多糖突变体的3种胞外酶活性和在含2%葡萄糖的NYGA培养基上的菌落形态的检测结果,将突变株分成5种类型。Southern杂交结果表明它们的突变位于基因组中的8个不同的位置,其中1个位于已鉴定的rpf调控基因簇。 |
| 关键词: 甘蓝黑腐病菌 胞外多糖 突变体 |
| DOI： |
| 投稿时间：1996-11-12修订日期：1996-12-13 |
| 基金项目:国家自然科学基金资助项目。(项目编号39100064)。 |
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| Classification of the Exopolysaccharide-deficient Mutants of Xanthomonas campestris pv. campestris Derived from Transposon Mutagenesis |
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Zha Dongxing, Tang Jiliang, Ma Qingsheng
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| (Laboratory of Molecular Genetics, Guangxi Agricultural University, 13 Xuiling Road, Nanning, Guangxi, 530005) |
| Abstract: |
| The 39 exopolysaccharide-deficient mutants of Xanthomonas campestris pv. campestris, which were derived from the transposon Tn5gusA5 mutagenesis, were divided into five groups by their activities of three extracellular enzymes and colony-phenotypes on the NYGA plates containing 2% glucose. Southern hybridization analysis revealed that their mutation sites were located respectively in eight different positions of the genome and one of them was located in the identified rpf regulatory gene cluster. |
| Key words: Xanthomonas campestris pv.campestris exopolysaccharide mutant |
