| 摘要: |
| 目前使用的多种疫苗仍未能有效控制鸡传染性法氏囊病(IBD)的发生,原因是疫苗质量难以保证和免疫程序或方法不当。解决的办法:病毒培养宜选用无特定病原(SPF)鸡胚或非免疫健康鸡群的鸡胚,消化时间控制在15min左右,细胞数在100~150万/mL,接毒量为0.1%~0.2%,小牛血清使用浓度为3%~5%;疫苗冻干过程应在不断搅拌病毒培养液的同时控制加入保护剂的速度。免疫程序:无母源抗体或母源抗体不清雏鸡,7日龄前首免,14和25日龄分别二次免疫;有母源抗体的雏鸡,10~14日龄首免,21日龄二免;流行严重地区,40日龄前后加强免疫1次。免疫方法为滴鼻、点眼。疫苗稀释用加0.5%脱脂奶粉的凉开水。最小免疫剂量为10万PFu,常规量为100万PFu。 |
| 关键词: 传染性法氏囊病病毒 疫苗 制造 使用 |
| DOI： |
| 投稿时间：1995-12-19 |
| 基金项目: |
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| On the Production and Application of the Vaccine of the Infectious Bursal Disease Virus |
|
Zhang Hongxing, Wu Baocheng
|
| (The Veterina et Zootechnica Department, Guangxi Argricultural Univ., 13 Xiuling Road, Nanning, Guangxi, 530005) |
| Abstract: |
| At present, Infectious Bursal Disease (IBD) is widespread epidemic and results in serious damage to the poultry of China, although several kinds of vaccines are applied. It's the main reason that the quality of the vaccine and the order or method of the immunity are inappropriate. The existing problems and the effective resolving measures are put forward in the manufacturing operation and application of vaccine:Chick embryo without maternal antibody (MA) must be chosen, Time of digestion is about 15 minutes, Cell number is 1~1.5 million/mL, Dose of inoculated virus's seed is 0.1%-0.2% culture solution included 3%-5% serum of newborn calf, and the speed is dominated when the culture solution of virus and protective agent are mixed before freeze-dried. For young chickens without MA or little being known about MA, the immunity are executed respectively in 7, 14 and 25-old-day chickens, but for young chickens having MA in 10-14, 21 and 40-old-day respectively with nasal drops or eye drops. The dose of common practice of vaccine is one million PFu per chicken, the minimum is a hundred thousand PFu. |
| Key words: Infectious Bursal disease Virus (IBDV) vaccine production application |
