| 引用本文： |
-
周素芳,周德义,张承禄,邓勇,吴耀生.木菠萝凝集素的纯化与性质研究[J].广西科学,1996,3(1):35-38. [点击复制]
- Zhou Sufang,zhou Deyi,Zhang Chenglu,Deng Yong,Wu Yaosheng.Purification and Property of Lectins from the Jack Fruit (Artocarpus heterophyllus)[J].Guangxi Sciences,1996,3(1):35-38. [点击复制]
|
|
| 摘要: |
| 木菠萝种子经抽提、40%~60%饱和度硫酸铵沉淀和Gal-Sepharose6B亲和层析,得到纯化的木菠萝凝集素(Jacalin)。Jacalin分子量约为58200,由分子量分别是14000和15800的亚基组成,等电聚焦电泳显示多条区带,其pI为5.7~8.65,Schiff's试剂染色证明它是一种糖蛋白,N-末端为精氨酸和甘氨酸。Jacalin能凝集除山羊、鸡以外的多种动物和人A、B、O和AB血型红细胞,凝集活性受N-酰凝半乳糖胺(GalNAc)和半乳糖(Gal)抑制。在pH值为4.5~10,pH改变不影响Jacalin凝集活性。 |
| 关键词: 木菠萝凝集素 纯化 性质 |
| DOI： |
| 投稿时间：1995-10-04 |
| 基金项目:国家自然科学基金资助项目(项目编号39260023)。 |
|
| Purification and Property of Lectins from the Jack Fruit (Artocarpus heterophyllus) |
|
Zhou Sufang, zhou Deyi, Zhang Chenglu, Deng Yong, Wu Yaosheng
|
| (Department of Biochemistry, Guangxi Medical University, 6 Binhu Road, Nanning, Guangxi, 530021) |
| Abstract: |
| The lectin of Jack Fruit (Jacalin) was isolated and purified from the seeds of the Jack Fruit by precipitate of 40%~60% saturation ammonium sulfate and affinity chromatography on Gal-Sepharose 6B. Jacalin had an apparent molecular weight of about 58 200 and is composed of two kinds of subunits which had molecular weight 14 000 and 15 800, respectively. Isoelectric focusing on polyacrylamide gel showed that Jacalin gave a spread of components with calculated isoelectric point between 5.7 and 8.65. Jacalin is a glycoprotein by assay of Schiff's stain. The two N-terminals are argenine and glynine. Jacalin could agglutinate red blood cells. |
| Key words: lectin of Jack Fruit purification property |
