| 引用本文： |
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邓卓霖,潘朗星,马韵,彭H. Z..肝细胞性肝癌P53基因多态性定点突变[J].广西科学,1995,2(4):59-60. [点击复制]
- Deng Zhuolin,Pan Langxing,Ma Yun,Peng H. Z..P53 Gene Polymorphic Point Mutation in Hepatocellular Carcinoma[J].Guangxi Sciences,1995,2(4):59-60. [点击复制]
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| 摘要: |
| 对P53阳性的8例肝细胞性肝癌(HCC)广西科学,1995,2(1):55~57)中的7例的P53基因进行DNA单键构象多态性分析(SSCP)和PCR扩增物直接测序。结果,7例均在P53第7外显子第249编码区的3号位发现异常。1例在249编码区的第3碱基微小丧失引起乱码突变;另6例在此位点同时出现突变的T和C带,成为G→(CT)的多态性突变形式。外显子5、6、8和9没有异常。估计除黄曲霉毒素B1外可能有别的致癌因素协同作用造成此种多态性。认为南宁地区是继中国江苏启东和莫桑比克后的第3个HCCP53基因249编码区有集中突变热点的地区。 |
| 关键词: P53基因 多态性突变 肝细胞性肝癌 |
| DOI： |
| 投稿时间：1995-09-07 |
| 基金项目: |
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| P53 Gene Polymorphic Point Mutation in Hepatocellular Carcinoma |
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Deng Zhuolin, Pan Langxing, Ma Yun, Peng H. Z.
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| (Guangxi Medical University, 6 Binhu Road, Nanning, Guangxi, 530021) |
| Abstract: |
| P53 gene DNA of 7 cases of 8 hepatocellular carcinomas(HCC) with positive P53 mutation (Guangxi Sciences, 1995, 2 (1):55~57) were detected by and proceeded for PCR-SSCP analysis and DNA direct sequencing. 7 HCCs showed a single point abnormality at position 3 of exon 7 codon 249. One showed a microdelection at position 3 of codon 249 leading to a frame shift, othersix showed two bands for mutants T & C at the third base of codon 249, leading to a G→(CT) polymorphic mutation form. No other mutation was in exon 5, 6, 8 and 9. There may be more other factors besides aftaloxin B1 for these polymorphic mutations. Nanning prefecture could be confirmed as a third area in the world with mutational hot-spot at canon 249 in the P53 gene in HCC. |
| Key words: P53 gene polymorphic mutation hepatocellular carcinoma |
