| 摘要: |
| 从毛花猕猴桃(Actinidia eriantha Benth)田间生长的茎段、种子无菌条件下萌发的下胚轴及试管苗的茎段和叶片得到愈伤组织。田间来源的茎段诱导愈伤组织较难,愈伤组织生长缓慢;下胚轴及试管苗茎段和叶片容易产生愈伤组织,愈伤组织生长旺盛。消毒时间、接种方法、基因型和培养基都可能影响到田间来源的茎段愈伤组织产生。在MS附加玉米素或6-苄氨基嘌呤(BAP)的培养基上下胚轴来源的愈伤组织不经转代即分化芽和根。试管苗茎段和叶片在附加玉米素或N-(2-氯基-4-吡啶基)-N'-苯基脲(CPPU)的MS培养基上培养一代也分化出芽、试管苗茎段在附加0.0025mg/LCPPU和0.1mg/L吲跺乙酸(IAA)的MS培养基上产生愈伤组织、芽的分化和苗生长都较理想;试管苗叶片则以附加0.025mg/LCPPU和0.1mg/LIAA或0.5mg/L玉米素和0.1mg/LIAA的MS培养基较好。当苗伸长至1cm或以上时切下,转入MS基本培养基(大量元素减半)上后可形成完整植株。 |
| 关键词: 毛花猕猴桃 愈伤组织 植株再生 |
| DOI： |
| 投稿时间：1994-10-31 |
| 基金项目: |
|
| Callus Induction and Plant Regeneration from Actinidia eriantha Benth |
|
Zhang Yuanji, Qian Yingqian
|
| (Institute of Botany, Academia Sinica, Beijing, 100044) |
| Abstract: |
| Calli were induced from stem segments of field-grown plants, elongated hypocotyls, stem segments and leaves of in vitro seedlings of A. eriantha. Callus induction from hypocotyls and explants of in vitro seedlings was much easier and calli grew vigorously, and that from field-grown stem segments was difficult and calli grew slowly. Factors affecting callus induction from field-grown stem segments were found to be disinfection duration, genotypes, and growth regulators used. Adventitious buds and roots differentiated from hypocotyl-derived calli on MS medium supplemented with zeatin or benzyl-aminopurine (BAP) without subculture. Bud and occasionally root differentiation also occured from in vitro seedlings on MS medium supplemented with zeatin or N-(2-chloro-4-pyridyl) -N'-phenylurea (CPPU) with one passage culture. The optimal media for the callus induction, bud differentiation and shoot growth to the stem segments of in vitro seedlings were MS medium supplemented with 0.0025mg/L. CPPU and 0.1 mg/L indole-3-acetic acid (IAA) and those to the leaves of in vitro seedlings were MS medium supplemented with 0.025 mg/L. CPPU and 0.1 mg/L IAA or 0.5 mg/L zeatin and 0.1 mg/L. IAA. Whole plants regenerated from shoots which were 1 cm in length or longer after transferring onto MS medium with half strength of macro-elements. |
| Key words: Actinidia eriantha callus induction whole plant regeneration |
