| 引用本文： |
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龙桂芳,李卫,林伟雄,金琪,谢健生,唐智宁.应用PCR-斑点杂交方法进行β地中海贫血产前基因诊断[J].广西科学,1994,1(2):1-4. [点击复制]
- Long Guifang,Li Wei,Lin Weixiong,Jin Qi,Xie Jiensheng,Tang Zhining.The Prenatal Gene Diagnoses of β-thalassaemia by PCR-dot-blotting[J].Guangxi Sciences,1994,1(2):1-4. [点击复制]
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| 摘要: |
| 从1989年以来,我们应用聚合酶链反应(PCR)和等位基因特异寡核苷酸斑点杂交(ASO-dot-blotting)技术对60例β地中海贫血高危胎儿进行产前基因诊断。结果查出β地中海贫血纯合子6例、双重杂合子8例、杂合子27例.其余19例为正常胎儿。PCR-dot-blotting方法准确可靠,尤其适合于β地中海贫血的产前诊断。 |
| 关键词: 聚合酶链反应(PCR) β地中海贫血 产前诊断 |
| DOI： |
| 投稿时间：1994-03-07 |
| 基金项目: |
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| The Prenatal Gene Diagnoses of β-thalassaemia by PCR-dot-blotting |
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Long Guifang, Li Wei, Lin Weixiong, Jin Qi, Xie Jiensheng, Tang Zhining
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| (Laboratory of Haemoglobin, Department of Pediatrics, Guangxi Medical University, 6 BinHu Road, Nanning, Guangxi, 530021) |
| Abstract: |
| Prenatal gene diagnoses on 60 fetuses at risk of β-thalassaemia major by PCR-dot-blotting have been performed since 1989.6 β-thalassaemia homozygotes,8 double heterozygotes and 27 heterozygotes were detected and,others were normal fetuses.PCR-dot-blotting was proved accurate and reliable,and especially applies to the prenatal gene diagnoses of β-thalassaemia. |
| Key words: polymerase chain reaction (PCR) β-thalassaemia prenatal gene diagnoses |
